Some Like it High! Phylogenetic Diversity of High-Elevation Cyanobacterial Community from Biological Soil Crusts of Western Himalaya
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26552394
DOI
10.1007/s00248-015-0694-4
PII: 10.1007/s00248-015-0694-4
Knihovny.cz E-zdroje
- Klíčová slova
- Cyanobacterial diversity, Desert, High-elevation, Phosphorus, Soil crusts, Western Himalayas,
- MeSH
- biodegradace MeSH
- biodiverzita * MeSH
- fylogeneze * MeSH
- geologické sedimenty analýza mikrobiologie MeSH
- molekulární sekvence - údaje MeSH
- nadmořská výška MeSH
- půda chemie MeSH
- půdní mikrobiologie * MeSH
- sinice klasifikace genetika izolace a purifikace metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Tibet MeSH
- Názvy látek
- půda MeSH
The environment of high-altitudinal cold deserts of Western Himalaya is characterized by extensive development of biological soil crusts, with cyanobacteria as dominant component. The knowledge of their taxonomic composition and dependency on soil chemistry and elevation is still fragmentary. We studied the abundance and the phylogenetic diversity of the culturable cyanobacteria and eukaryotic microalgae in soil crusts along altitudinal gradients (4600-5900 m) at two sites in the dry mountains of Ladakh (SW Tibetan Plateau and Eastern Karakoram), using both microscopic and molecular approaches. The effects of environmental factors (altitude, mountain range, and soil physico-chemical parameters) on the composition and biovolume of phototrophs were tested by multivariate redundancy analysis and variance partitioning. Both phylogenetic diversity and composition of morphotypes were similar between Karakorum and Tibetan Plateau. Phylogenetic analysis of 16S rRNA gene revealed strains belonging to at least five genera. Besides clusters of common soil genera, e.g., Microcoleus, Nodosilinea, or Nostoc, two distinct clades of simple trichal taxa were newly discovered. The most abundant cyanobacterial orders were Oscillatoriales and Nostacales, whose biovolume increased with increasing elevation, while that of Chroococales decreased. Cyanobacterial species richness was low in that only 15 morphotypes were detected. The environmental factors accounted for 52 % of the total variability in microbial data, 38.7 % of which was explained solely by soil chemical properties, 14.5 % by altitude, and 8.4 % by mountain range. The elevation, soil phosphate, and magnesium were the most important predictors of soil phototrophic communities in both mountain ranges despite their different bedrocks and origin. The present investigation represents a first record on phylogenetic diversity of the cyanobacterial community of biological soil crusts from Western Himalayas and first record from altitudes over 5000 m.
Faculty of Science University of South Bohemia Branišovská 31 České Budějovice 37005 Czech Republic
Institute of Hydrobiology Biology Centre of AS CR Na Sádkách 7 České Budějovice 37005 Czech Republic
Zobrazit více v PubMed
Folia Microbiol (Praha). 2004;49(2):105-11 PubMed
Syst Biol. 2012 May;61(3):539-42 PubMed
Mol Biol Evol. 2013 Apr;30(4):772-80 PubMed
Science. 2007 Jul 6;317(5834):58-62 PubMed
Proc Biol Sci. 2008 Dec 22;275(1653):2793-802 PubMed
Environ Microbiol. 2013 Sep;15(9):2505-16 PubMed
Mol Biol Evol. 2010 Feb;27(2):221-4 PubMed
Appl Environ Microbiol. 2003 Sep;69(9):5157-69 PubMed
FEBS Lett. 1993 Feb 8;317(1-2):96-100 PubMed
Proc Natl Acad Sci U S A. 2006 Jan 17;103(3):626-31 PubMed
Microb Ecol. 2005 Oct;50(3):396-407 PubMed
Appl Environ Microbiol. 1997 Aug;63(8):3327-32 PubMed
Microb Ecol. 2011 Aug;62(2):337-46 PubMed
PLoS One. 2013;8(1):e53514 PubMed
Environ Microbiol. 2010 Jul;12(7):1842-54 PubMed
Nature. 2004 May 13;429(6988):174-7 PubMed
Syst Biol. 2010 May;59(3):307-21 PubMed
Nat Methods. 2012 Jul 30;9(8):772 PubMed
