Some Like it High! Phylogenetic Diversity of High-Elevation Cyanobacterial Community from Biological Soil Crusts of Western Himalaya
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26552394
DOI
10.1007/s00248-015-0694-4
PII: 10.1007/s00248-015-0694-4
Knihovny.cz E-resources
- Keywords
- Cyanobacterial diversity, Desert, High-elevation, Phosphorus, Soil crusts, Western Himalayas,
- MeSH
- Biodegradation, Environmental MeSH
- Biodiversity * MeSH
- Phylogeny * MeSH
- Geologic Sediments analysis microbiology MeSH
- Molecular Sequence Data MeSH
- Altitude MeSH
- Soil chemistry MeSH
- Soil Microbiology * MeSH
- Cyanobacteria classification genetics isolation & purification metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Tibet MeSH
- Names of Substances
- Soil MeSH
The environment of high-altitudinal cold deserts of Western Himalaya is characterized by extensive development of biological soil crusts, with cyanobacteria as dominant component. The knowledge of their taxonomic composition and dependency on soil chemistry and elevation is still fragmentary. We studied the abundance and the phylogenetic diversity of the culturable cyanobacteria and eukaryotic microalgae in soil crusts along altitudinal gradients (4600-5900 m) at two sites in the dry mountains of Ladakh (SW Tibetan Plateau and Eastern Karakoram), using both microscopic and molecular approaches. The effects of environmental factors (altitude, mountain range, and soil physico-chemical parameters) on the composition and biovolume of phototrophs were tested by multivariate redundancy analysis and variance partitioning. Both phylogenetic diversity and composition of morphotypes were similar between Karakorum and Tibetan Plateau. Phylogenetic analysis of 16S rRNA gene revealed strains belonging to at least five genera. Besides clusters of common soil genera, e.g., Microcoleus, Nodosilinea, or Nostoc, two distinct clades of simple trichal taxa were newly discovered. The most abundant cyanobacterial orders were Oscillatoriales and Nostacales, whose biovolume increased with increasing elevation, while that of Chroococales decreased. Cyanobacterial species richness was low in that only 15 morphotypes were detected. The environmental factors accounted for 52 % of the total variability in microbial data, 38.7 % of which was explained solely by soil chemical properties, 14.5 % by altitude, and 8.4 % by mountain range. The elevation, soil phosphate, and magnesium were the most important predictors of soil phototrophic communities in both mountain ranges despite their different bedrocks and origin. The present investigation represents a first record on phylogenetic diversity of the cyanobacterial community of biological soil crusts from Western Himalayas and first record from altitudes over 5000 m.
Faculty of Science University of South Bohemia Branišovská 31 České Budějovice 37005 Czech Republic
Institute of Hydrobiology Biology Centre of AS CR Na Sádkách 7 České Budějovice 37005 Czech Republic
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