Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
- Klíčová slova
- 5-Ethynyl-2′deoxyuridine, Cell division, Cell proliferation, DNA labeling, DNA replication, EdU, Histology,
- MeSH
- Arabidopsis cytologie ultrastruktura MeSH
- barvení a značení metody MeSH
- deoxyuridin analogy a deriváty analýza MeSH
- DNA rostlinná analýza MeSH
- kořeny rostlin ultrastruktura MeSH
- meristém ultrastruktura MeSH
- proliferace buněk * MeSH
- replikace DNA MeSH
- rostlinné buňky ultrastruktura MeSH
- semenáček ultrastruktura MeSH
- zalévání tkání do parafínu metody MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 5-ethynyl-2'-deoxyuridine MeSH Prohlížeč
- deoxyuridin MeSH
- DNA rostlinná MeSH
The ability to analyze cell division in both spatial and temporal dimensions within an organism is a key requirement in developmental biology. Specialized cell types within individual organs, such as those within shoot and root apical meristems, have often been identified by differences in their rates of proliferation prior to the characterization of distinguishing molecular markers. Replication-dependent labeling of DNA is a widely used method for assaying cell proliferation. The earliest approaches used radioactive labeling with tritiated thymidine, which were later followed by immunodetection of bromodeoxyuridine (BrdU). A major advance in DNA labeling came with the use of 5-ethynyl-2'deoxyuridine (EdU) which has proven to have multiple advantages over BrdU. Here we describe the methodology for analyzing EdU labeling and retention in whole plants and histological sections of Arabidopsis.
Citace poskytuje Crossref.org
Visualization of the Nucleolus Using Ethynyl Uridine