Increased serum levels of C21 steroids in female patients with multiple sclerosis
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26680486
DOI
10.33549/physiolres.933145
PII: 933145
Knihovny.cz E-resources
- MeSH
- Biomarkers blood MeSH
- Adult MeSH
- gamma-Aminobutyric Acid blood MeSH
- Middle Aged MeSH
- Humans MeSH
- Gas Chromatography-Mass Spectrometry methods MeSH
- Receptors, GABA-A blood MeSH
- Multiple Sclerosis blood diagnosis MeSH
- Steroids blood MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- gamma-Aminobutyric Acid MeSH
- Receptors, GABA-A MeSH
- Steroids MeSH
Multiple sclerosis (MS) is one of the most common neurological diseases. This neurodegenerative autoimmune disease manifests as inflammatory and demyelinating impairment of the central nervous system (CNS). Although some studies demonstrated associations between altered steroidogenesis and pathophysiology of MS as well as the importance of steroids in the pathophysiology of MS, the knowledge concerning the steroid metabolome in female patients is limited. Hence, 51 steroids and steroid polar conjugates were measured in the serum of 12 women with MS, untreated with steroids and 6 age-corresponding female controls with the use of gas chromatography - mass spectrometry (GC-MS). The data were processed using age adjusted ANCOVA, receiver operating characteristics (ROC) analysis and orthogonal projections to latent structures (OPLS). Our data show higher levels of circulating C21 steroids including steroid modulators of ionotropic type A gamma-aminobutyric acid (GABA A) receptors and glutamate receptors. Furthermore, the levels of GABAergic androsterone and 5-androsten-3beta,7alpha,17beta-triol were also higher in the female MS patients. In conclusion, the data demonstrate higher levels of circulating C21 steroids and their polar conjugates and some bioactive C19 steroids in women with MS, which may influence neuronal activity and affect the balance between neuroprotection and excitotoxicity.
References provided by Crossref.org
Altered Steroidome in Women with Multiple Sclerosis
Steroid Sulfation in Neurodegenerative Diseases
Analytical Methods for the Determination of Neuroactive Steroids