In vitro synergistic effect of Hibiscus sabdariffa aqueous extract in combination with standard antibiotics against Helicobacter pylori clinical isolates
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
- Keywords
- Antimicrobial agents, H. pylori infection, combinatory effect, drug-resistant strains, natural products,
- MeSH
- Amoxicillin pharmacology MeSH
- Anti-Bacterial Agents isolation & purification pharmacology MeSH
- Phytotherapy MeSH
- Helicobacter pylori drug effects growth & development isolation & purification MeSH
- Hibiscus chemistry MeSH
- Helicobacter Infections drug therapy microbiology MeSH
- Clarithromycin pharmacology MeSH
- Drug Therapy, Combination MeSH
- Flowers MeSH
- Plants, Medicinal MeSH
- Humans MeSH
- Metronidazole pharmacology MeSH
- Microbial Sensitivity Tests MeSH
- Plant Extracts isolation & purification pharmacology MeSH
- Drug Synergism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Amoxicillin MeSH
- Anti-Bacterial Agents MeSH
- Clarithromycin MeSH
- Metronidazole MeSH
- Plant Extracts MeSH
Context The increasing problem of drug-resistant strains has led to the failure of current treatment regimens of Helicobacter pylori (HP) infection. Recently, a new treatment strategy has been developed to overcome the problem by using natural products in combination with antibiotics to enhance the treatment efficacy. Objective The antimicrobial combinatory effect of the aqueous extract of Hibiscus sabdariffa L. (Malvaceae) (AEHS) with antibiotics (clarithromycin, CLA; amoxicillin, AMX; metronidazole, MTZ) has been evaluated in vitro against HP strains. Materials and methods Hibiscus calyces (35 g) were brewed in 250 mL of boiled water for 30 min, and minimum inhibitory concentrations (MICs) were determined by agar dilution method. The checkerboard assay was used to evaluate the antimicrobial combinatory effect according to the sum of fractional inhibitory concentration (∑FIC) indices. Results In this study, AEHS exerted remarkable bacteriostatic effect against all HP strains tested with MICs values ranging from 9.18 to 16.68 μg/mL. Synergy effect of AEHS with CLA or MTZ was obtained against four of seven HP strains tested with ∑FIC ranging from 0.21 to 0.39. The additive effect of AEHS with AMX was obtained against five of seven HP strains tested with ∑FIC ranging from 0.61 to 0.91. Conclusion This study presents AEHS as a potent therapeutic candidate alone, or in combination with antibiotics for the treatment of HP infection.
References provided by Crossref.org
