High frequency of Candida fabianii among clinical isolates biochemically identified as Candida pelliculosa and Candida utilis
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
PubMed
26763103
DOI
10.1111/myc.12454
Knihovny.cz E-zdroje
- Klíčová slova
- Candida fabianii, MALDI-TOF MS, antifungal susceptibility testing, biochemical identification,
- MeSH
- antifungální látky farmakologie MeSH
- Candida klasifikace izolace a purifikace MeSH
- dítě MeSH
- dospělí MeSH
- kandidóza epidemiologie mikrobiologie MeSH
- kojenec MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mykologické určovací techniky MeSH
- nemocnice MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- prevalence MeSH
- prospektivní studie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- kojenec MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
- Názvy látek
- antifungální látky MeSH
Clinical yeast isolates belonging to Candida pelliculosa, Candida utilis and Candida fabianii are difficult to distinguish in a routine mycology laboratory using common biochemical tests. The aims of this study were to determine the prevalence of C. pelliculosa, C. utilis and C. fabianii in clinical samples and to compare their minimum inhibitory concentrations (MICs) to systemic antifungals. Two hundred and forty-eight clinical yeast isolates obtained from eight large hospitals in the Czech Republic were included in this study. Identification was performed biochemically using ID 32C kit and by MALDI-TOF MS. MICs were determined using colorimetric broth dilution Sensititre YeastOne panels. From a total number of 248 isolates, 175 were identified as C. pelliculosa and 73 as C. utilis using the biochemical kit. In contrast, MALDI-TOF MS identified 222 isolates as C. fabianii, 20 as C. pelliculosa and 6 as C. utilis. The highest mean MICs were found in C. fabianii and, regardless of the studied species, in isolates from blood cultures and central venous catheters. MALDI-TOF MS revealed C. fabianii to be most prevalent in clinical samples as compared with the other studied species. Higher MIC values in C. fabianii support the importance of correct identification of this species.
Department of Clinical Microbiology University Hospital Brno Brno Czech Republic
Department of Microbiology Faculty of Medicine Masaryk University Brno Czech Republic
Laboratory of Clinical Mycology Public Health Institute Ostrava Ostrava Czech Republic
Laboratory of Parasitology and Mycology Ceske Budejovice Hospital Ceske Budejovice Czech Republic
Research and Development Department GeneProof a s Brno Czech Republic
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