High frequency of Candida fabianii among clinical isolates biochemically identified as Candida pelliculosa and Candida utilis
Language English Country Germany Media print-electronic
Document type Journal Article
PubMed
26763103
DOI
10.1111/myc.12454
Knihovny.cz E-resources
- Keywords
- Candida fabianii, MALDI-TOF MS, antifungal susceptibility testing, biochemical identification,
- MeSH
- Antifungal Agents pharmacology MeSH
- Candida classification isolation & purification MeSH
- Child MeSH
- Adult MeSH
- Candidiasis epidemiology microbiology MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Adolescent MeSH
- Young Adult MeSH
- Mycological Typing Techniques MeSH
- Hospitals MeSH
- Infant, Newborn MeSH
- Child, Preschool MeSH
- Prevalence MeSH
- Prospective Studies MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Infant, Newborn MeSH
- Child, Preschool MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- Antifungal Agents MeSH
Clinical yeast isolates belonging to Candida pelliculosa, Candida utilis and Candida fabianii are difficult to distinguish in a routine mycology laboratory using common biochemical tests. The aims of this study were to determine the prevalence of C. pelliculosa, C. utilis and C. fabianii in clinical samples and to compare their minimum inhibitory concentrations (MICs) to systemic antifungals. Two hundred and forty-eight clinical yeast isolates obtained from eight large hospitals in the Czech Republic were included in this study. Identification was performed biochemically using ID 32C kit and by MALDI-TOF MS. MICs were determined using colorimetric broth dilution Sensititre YeastOne panels. From a total number of 248 isolates, 175 were identified as C. pelliculosa and 73 as C. utilis using the biochemical kit. In contrast, MALDI-TOF MS identified 222 isolates as C. fabianii, 20 as C. pelliculosa and 6 as C. utilis. The highest mean MICs were found in C. fabianii and, regardless of the studied species, in isolates from blood cultures and central venous catheters. MALDI-TOF MS revealed C. fabianii to be most prevalent in clinical samples as compared with the other studied species. Higher MIC values in C. fabianii support the importance of correct identification of this species.
Department of Clinical Microbiology University Hospital Brno Brno Czech Republic
Department of Microbiology Faculty of Medicine Masaryk University Brno Czech Republic
Laboratory of Clinical Mycology Public Health Institute Ostrava Ostrava Czech Republic
Laboratory of Parasitology and Mycology Ceske Budejovice Hospital Ceske Budejovice Czech Republic
Research and Development Department GeneProof a s Brno Czech Republic
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