BACKGROUND AND OBJECTIVES: The probiotic bacterium Escherichia coli strain Nissle 1917 has previously been shown to alter the pharmacokinetics of amiodarone. The aim of this study was to determine whether the probiotic bacterium Lactobacillus casei produces similar alterations in amiodarone disposition. METHODS: A suspension of live probiotic bacteria L. casei strain DN-114 001 (1.5 × 109 CFU/dose; probiotic pre-treated group) or a saline solution (control group) was administered directly into the stomach of male Wistar rats (N = 30 in each group) by oral gavage daily for 7 consecutive days. On the eighth day, all rats (N = 60) were given a single oral dose of an amiodarone hydrochloride suspension (model drug; 50 mg/kg). The concentrations of amiodarone and of its main metabolite N-desethylamiodarone were determined in rat plasma by high-performance liquid chromatography. RESULTS: Comparison of the pharmacokinetics of amiodarone in the control group and probiotic pre-treated group revealed that the peak plasma concentration of amiodarone was delayed by >2 h in the probiotic pre-treated group. The plasma level of N-desethylamiodarone was unchanged in the probiotic pre-medicated group and its pharmacokinetic parameters were not altered. CONCLUSIONS: The slower absorption of amiodarone in the probiotic pre-treated rats compared to the control ones and the unchanged pharmacokinetics of its main metabolite suggest that the probiotic strain of L. casei DN-114 001 has probably no clinical consequences as the difference was not statistically significant.

Department of Medical Chemistry and Biochemistry Faculty of Medicine and Dentistry Palacky University Olomouc Hnevotinska 3 775 15 Olomouc Czech Republic

Department of Pharmacology Faculty of Medicine and Dentistry Palacky University Olomouc Hnevotinska 3 775 15 Olomouc Czech Republic

Institute of Microbiology Academy of Sciences of the Czech Republic Videnska 1083 142 20 Prague Czech Republic

Institute of Molecular and Translational Medicine Faculty of Medicine and Dentistry Palacky University Olomouc Hnevotinska 5 779 00 Olomouc Czech Republic

Zobrazit více v PubMed

Phytother Res. 2015 Jun;29(6):825-34 PubMed

Am J Physiol Gastrointest Liver Physiol. 2011 Jun;300(6):G1115-23 PubMed

Am Fam Physician. 2003 Dec 1;68(11):2189-96 PubMed

Pharmacol Ther. 2015 May;149:1-123 PubMed

Curr Issues Mol Biol. 2014;16:55-68 PubMed

J Vet Pharmacol Ther. 2015 Dec;38(6):549-55 PubMed

Arch Drug Inf. 2008 Jul;1(1):35-41 PubMed

Drug Metab Dispos. 2000 Nov;28(11):1303-10 PubMed

J Pharm Pharm Sci. 2008;11(1):147-59 PubMed

Microb Cell Fact. 2014 Aug 29;13 Suppl 1:S9 PubMed

J Pharm Pharm Sci. 2001 Sep-Dec;4(3):263-8 PubMed

Eur J Drug Metab Pharmacokinet. 2008 Apr-Jun;33(2):101-6 PubMed

Phytother Res. 2015 May;29(5):785-90 PubMed

PLoS One. 2014 Feb 05;9(2):e87150 PubMed

Ther Drug Monit. 2012 Aug;34(4):467-76 PubMed

Eur J Clin Pharmacol. 2000 Nov;56(8):523-4 PubMed

Br J Clin Pharmacol. 1998 Aug;46(2):101-10 PubMed

J Membr Biol. 1997 Dec 1;160(3):161-75 PubMed