Transcripts of genes encoding reproductive neuroendocrine hormones and androgen receptor in the brain and testis of goldfish exposed to vinclozolin, flutamide, testosterone, and their combinations
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
26899179
DOI
10.1007/s10695-016-0205-7
PII: 10.1007/s10695-016-0205-7
Knihovny.cz E-zdroje
- Klíčová slova
- Androgen receptor, Flutamide, Kisspeptin, Luteinizing hormone receptor, Salmon gonadotropin-releasing hormone, Testosterone,
- MeSH
- androgenní receptory genetika MeSH
- androgeny farmakologie MeSH
- antagonisté androgenů farmakologie MeSH
- flutamid farmakologie MeSH
- hormon uvolňující gonadotropiny genetika MeSH
- karas zlatý genetika MeSH
- kisspeptiny genetika MeSH
- kyselina pyrrolidonkarboxylová analogy a deriváty MeSH
- lékové interakce MeSH
- messenger RNA metabolismus MeSH
- mozek účinky léků metabolismus MeSH
- oxazoly farmakologie MeSH
- průmyslové fungicidy farmakologie MeSH
- receptory LH genetika MeSH
- receptory spřažené s G-proteiny genetika MeSH
- rozmnožování účinky léků MeSH
- rybí proteiny genetika MeSH
- testis účinky léků metabolismus MeSH
- testosteron farmakologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- androgenní receptory MeSH
- androgeny MeSH
- antagonisté androgenů MeSH
- flutamid MeSH
- gonadotropin-releasing hormone-III MeSH Prohlížeč
- hormon uvolňující gonadotropiny MeSH
- kisspeptiny MeSH
- kyselina pyrrolidonkarboxylová MeSH
- messenger RNA MeSH
- oxazoly MeSH
- průmyslové fungicidy MeSH
- receptory LH MeSH
- receptory spřažené s G-proteiny MeSH
- rybí proteiny MeSH
- testosteron MeSH
- vinclozolin MeSH Prohlížeč
Vinclozolin (VZ) is a pesticide that acts as an anti-androgen to impair reproduction in mammals. However, VZ-induced disruption of reproduction is largely unknown in fish. In the present study, we have established a combination exposure in which adult goldfish were exposed to VZ (30 and 100 μg/L), anti-androgen flutamide (Flu, 300 μg/L), and androgen testosterone (T, 1 μg/L) to better understand effects of VZ on reproductive endocrine system. mRNA levels of kisspeptin (kiss-1 and kiss-2) and its receptor (gpr54), salmon gonadotropin-releasing hormone (gnrh3) and androgen receptor (ar) in the mid-brain, and luteinizing hormone receptor (lhr) in the testis were analyzed and compared with those of control following 10 days of exposure. kiss-1 mRNA level was increased in goldfish exposed to 100 µg/L VZ and to Flu, while kiss-2 mRNA level was increased following exposure to Flu and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. gpr54 mRNA level was increased in goldfish exposed to Flu and to combination of 30 µg/L VZ with Flu and 100 µg/L VZ with T. gnrh3 mRNA level was increased in goldfish exposed to 100 µg/L VZ, to Flu, and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. The mid-brain ar mRNA level was increased in goldfish exposed to Flu and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. Testicular lhr mRNA level was increased in goldfish exposed to Flu and to combination of 30 µg/L VZ with Flu. These results suggest that VZ and Flu are capable of interfering with kisspeptin and GnRH systems to alter pituitary and testicular horonal functions in adult goldfish and the brain ar mediates VZ-induced disruption of androgen production.
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