Characterization of tet(Y)-carrying LowGC plasmids exogenously captured from cow manure at a conventional dairy farm
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
27083193
DOI
10.1093/femsec/fiw075
PII: fiw075
Knihovny.cz E-zdroje
- Klíčová slova
- Acinetobacter, LowGC plasmids, cattle manure, horizontal gene transfer, tet(Y), tetracycline resistance,
- MeSH
- Acinetobacter účinky léků genetika MeSH
- antibakteriální látky farmakologie MeSH
- chlortetracyklin farmakologie MeSH
- farmy MeSH
- hnůj analýza MeSH
- mapování chromozomů MeSH
- plazmidy genetika MeSH
- prasata MeSH
- půda MeSH
- půdní mikrobiologie MeSH
- rezistence na tetracyklin genetika MeSH
- sekvence nukleotidů genetika MeSH
- sekvenční analýza DNA MeSH
- skot MeSH
- streptomycin farmakologie MeSH
- zastoupení bazí genetika MeSH
- zemědělství MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
- chlortetracyklin MeSH
- hnůj MeSH
- půda MeSH
- streptomycin MeSH
Manure from dairy farms has been shown to contain diverse tetracycline resistance genes that are transferable to soil. Here, we focus on conjugative plasmids that may spread tetracycline resistance at a conventional dairy farm. We performed exogenous plasmid isolation from cattle feces using chlortetracycline for transconjugant selection. The transconjugants obtained harbored LowGC-type plasmids and tet(Y). A representative plasmid (pFK2-7) was fully sequenced and this was compared with previously described LowGC plasmids from piggery manure-treated soil and a GenBank record from Acinetobacter nosocomialis that we also identified as a LowGC plasmid. The pFK2-7 plasmid had the conservative backbone typical of LowGC plasmids, though this region was interrupted with an insert containing the tet(Y)-tet(R) tetracycline resistance genes and the strA-strB streptomycin resistance genes. Despite Acinetobacter populations being considered natural hosts of LowGC plasmids, these plasmids were not found in three Acinetobacter isolates from the study farm. The isolates harbored tet(Y)-tet(R) genes in identical genetic surroundings as pFK2-7, however, suggesting genetic exchange between Acinetobacter and LowGC plasmids. Abundance of LowGC plasmids and tet(Y) was correlated in manure and soil samples from the farm, indicating that LowGC plasmids may be involved in the spread of tet(Y) in the environment.
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