Ex Vivo and In Vitro Studies on the Cytotoxicity and Immunomodulative Properties of Poly(2-isopropenyl-2-oxazoline) as a New Type of Biomedical Polymer
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- biocompatibility, cell proliferation, fluorescence, functionalization of polymers, poly(2-isopropenyl-2-oxazoline), splenocytes,
- MeSH
- Biocompatible Materials chemical synthesis chemistry pharmacology MeSH
- Cell Death drug effects MeSH
- 3T3 Cells MeSH
- Endocytosis drug effects MeSH
- Fibroblasts cytology MeSH
- Spectrometry, Fluorescence MeSH
- Immunomodulation drug effects MeSH
- Microscopy, Confocal MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Organelles drug effects metabolism MeSH
- Oxazoles chemical synthesis chemistry pharmacology MeSH
- Polymers chemical synthesis chemistry pharmacology MeSH
- Polypropylenes chemical synthesis chemistry pharmacology MeSH
- Cell Proliferation drug effects MeSH
- Spleen cytology MeSH
- Cell Survival drug effects MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biocompatible Materials MeSH
- Oxazoles MeSH
- poly(2-isopropenyl-2-oxazoline) MeSH Browser
- Polymers MeSH
- Polypropylenes MeSH
Poly(2-alkenyl-2-oxazoline)s are promising functional polymers for a variety of biomedical applications, such as drug delivery systems, peptide conjugates, or gene delivery. In this study, poly(2-isopropenyl-2-oxazoline) (PIPOx) is prepared through free-radical polymerization initiated with azobisisobutyronitrile. Reactive 2-oxazoline units in the side chain support an addition reaction with different compounds containing a carboxylic group, which facilitates the preparation of polymers labeled with two different fluorescent dyes. The cytotoxicities of 2-oxazoline monomers, PIPOx, and fluorescently labeled PIPOx are evaluated in vitro using an 3-(4,5-Dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and ex vivo using a cell proliferation assay with adenosine triphosphate bioluminescence. The cell uptake of labeled PIPOx is used to determine the colocalization of PIPOx with cell organelles that are part of the endocytic pathway. For the first time, it is shown that poly(2-isopropenyl-2-oxazoline) is a biocompatible material and is suitable for biomedical applications; further, its immunomodulative properties are evaluated.
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