Poly(2-alkenyl-2-oxazoline)s are promising functional polymers for a variety of biomedical applications, such as drug delivery systems, peptide conjugates, or gene delivery. In this study, poly(2-isopropenyl-2-oxazoline) (PIPOx) is prepared through free-radical polymerization initiated with azobisisobutyronitrile. Reactive 2-oxazoline units in the side chain support an addition reaction with different compounds containing a carboxylic group, which facilitates the preparation of polymers labeled with two different fluorescent dyes. The cytotoxicities of 2-oxazoline monomers, PIPOx, and fluorescently labeled PIPOx are evaluated in vitro using an 3-(4,5-Dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and ex vivo using a cell proliferation assay with adenosine triphosphate bioluminescence. The cell uptake of labeled PIPOx is used to determine the colocalization of PIPOx with cell organelles that are part of the endocytic pathway. For the first time, it is shown that poly(2-isopropenyl-2-oxazoline) is a biocompatible material and is suitable for biomedical applications; further, its immunomodulative properties are evaluated.

Centre of Polymer Systems University Institute Tomas Bata University in Zlin Trˇída T Bati 5678 Zlin 760 01 Czech Republic

Department for Biomaterials Research Polymer Institute Slovak Academy of Sciences Dubravska cesta 9 845 41 Bratislava Slovakia

Institute of Chemistry Center of Glycomics Slovak Academy of Sciences Dubravska cesta 9 84236 Bratislava Slovakia

Institute of Experimental Pharmacology and Toxicology Slovak Academy of Sciences Dubravska cesta 9 841 04 Bratislava Slovakia

International Laser Centre Ilkovičova 3 841 04 Bratislava Slovakia

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