Thin layers of chitosan (positively charged)/sodium hyaluronate (negatively charged)/nonwoven fabrics were constructed by polyelectrolyte multilayer pad-dry-cure technique. Pure chitosan (CS) was isolated from shrimp shell and immobilized onto nonwoven fabrics (NWFs) using citric acid (CTA) as cross linker and solvent agents through a pad-dry-cure method. The prepared thin layer of chitosan citrate/nonwoven fabrics (CSCTA/NWFs) were consequently impregnated with hyaluronan (CSCTA/HA/NWFs) in the second path through a pad-dry-cure method. Chitosan/hyaluronan/nonwoven fabrics wound dressing was characterized by different techniques such as FTIR-ATR, TGA and SEM. The antibacterial activity and the cytotoxicity of the dressing sheets were evaluated against Escherichia coli (E. coli) and Streptococcus aureus (S. aureus), mouse fibroblast (NIH-3T3) and keratinocytes (HaCaT) cell lines, respectively. The cell-fabrics interaction was also investigated using fluorescence microscope, based on live/dead staining assay of 3T3 cells. The healing properties of the new wound dressing were evaluated and compared with the control sample.

CEITEC Central European Institute of Technology Brno University of Technology Brno Czech Republic

CEITEC Central European Institute of Technology Brno University of Technology Brno Czech Republic; National Research Centre Textile Research Division Pre Treatment and Finishing of Cellulosic Fibers Dokki Cairo Egypt; Faculty of Chemistry Institute of Materials Chemistry Brno University of Technology Brno Czech Republic

CEITEC Central European Institute of Technology Brno University of Technology Brno Czech Republic; National Research Centre Textile Research Division Pre Treatment and Finishing of Cellulosic Fibers Dokki Cairo Egypt; SCITEG a s Brno Czech Republic

Chemistry Department Faculty of Science Fayoum University Fayoum Egypt

Department of Physical Electronics Masaryk University Brno Czech Republic

Institute of Chemistry and Technology of Macromolecular Compounds Faculty of Chemical Technology University of Pardubice Pardubice Czech Republic

Institute of Organic Chemistry and Technology Faculty of Chemical Technology University of Pardubice Pardubice Czech Republic

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