Promoter activities in Corynebacterium glutamicum strains with deletions of genes encoding sigma factors of RNA polymerase suggested that transcription from some promoters is controlled by two sigma factors. To prove that different sigma factors are involved in the recognition of selected Corynebacterium glutamicum promoters, in vitro transcription system was applied. It was found that a typical housekeeping promoter Pper interacts with the alternative sigma factor σ(B) in addition to the primary sigma factor σ(A). On the other way round, the σ(B)-dependent promoter of the pqo gene that is expressed mainly in the stationary growth phase was active also with σ(A). Some promoters of genes involved in stress responses (P1clgR, P2dnaK, and P2dnaJ2) were found to be recognized by two stress-responding sigma factors, σ(H) and σ(E). In vitro transcription system thus proved to be a useful direct technique for demonstrating the overlap of different sigma factors in recognition of individual promoters in C. glutamicum.

Institute of Microbiology Czech Academy of Sciences v v i Vídeňská 1083 142 20 Prague Czech Republic

See more in PubMed

Mol Microbiol. 2016 May;100(3):486-509 PubMed

Curr Microbiol. 2007 Sep;55(3):234-9 PubMed

FEMS Microbiol Lett. 2008 Feb;279(2):195-201 PubMed

FEMS Microbiol Rev. 2010 Sep;34(5):685-737 PubMed

Appl Microbiol Biotechnol. 1999 Oct;52(4):541-5 PubMed

Nat Struct Mol Biol. 2006 Sep;13(9):806-14 PubMed

J Bacteriol. 2009 Aug;191(15):4951-8 PubMed

EMBO J. 1990 Nov;9(11):3733-42 PubMed

Appl Microbiol Biotechnol. 2012 Oct;96(2):521-9 PubMed

J Bacteriol. 1997 Mar;179(5):1525-32 PubMed

BMC Genomics. 2012 Sep 03;13:445 PubMed

Mol Microbiol. 2004 Apr;52(1):285-302 PubMed

Microbiology. 2008 Mar;154(Pt 3):915-23 PubMed

J Bacteriol. 2007 Jul;189(13):4696-707 PubMed

J Bacteriol. 2001 Mar;183(6):1921-7 PubMed

J Bacteriol. 2009 May;191(9):2964-72 PubMed

Appl Environ Microbiol. 2008 Aug;74(16):5146-52 PubMed

J Biotechnol. 2011 Jul 10;154(2-3):101-13 PubMed

BMC Genomics. 2007 Jan 04;8:4 PubMed

Infect Immun. 2006 Apr;74(4):2457-61 PubMed

J Bacteriol. 2004 Jul;186(14):4813-7 PubMed

PLoS One. 2009 Oct 19;4(10):e7466 PubMed

J Mol Biol. 1986 May 5;189(1):113-30 PubMed

J Bacteriol. 2006 Feb;188(4):1341-50 PubMed

Overlapping SigH and SigE sigma factor regulons in Corynebacterium glutamicum

Overlap of Promoter Recognition Specificity of Stress Response Sigma Factors SigD and SigH in Corynebacterium glutamicum ATCC 13032

Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum