Validation of Minim typing for fast and accurate discrimination of extended-spectrum, beta-lactamase-producing Klebsiella pneumoniae isolates in tertiary care hospital
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
27394639
DOI
10.1016/j.diagmicrobio.2016.03.010
PII: S0732-8893(16)30050-5
Knihovny.cz E-resources
- Keywords
- ESBL, High-resolution melt analysis, Klebsiella pneumoniae, Minim typing, Multi-locus sequence typing,
- MeSH
- beta-Lactamases metabolism MeSH
- Time Factors MeSH
- Tertiary Care Centers MeSH
- Klebsiella Infections microbiology MeSH
- Klebsiella pneumoniae classification enzymology isolation & purification MeSH
- Humans MeSH
- Molecular Typing methods MeSH
- Polymerase Chain Reaction MeSH
- Carrier State microbiology MeSH
- Sequence Analysis, DNA MeSH
- Transition Temperature MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- beta-Lactamases MeSH
Minim typing is derived from the multi-locus sequence typing (MLST). It targets the same genes, but sequencing is replaced by high resolution melt analysis. Typing can be performed by analysing six loci (6MelT), four loci (4MelT) or using data from four loci plus sequencing the tonB gene (HybridMelT). The aim of this study was to evaluate Minim typing to discriminate extended-spectrum beta-lactamase producing Klebsiella pneumoniae (ESBL-KLPN) isolates at our hospital. In total, 380 isolates were analyzed. The obtained alleles were assigned according to both the 6MelT and 4MelT typing scheme. In 97 isolates, the tonB gene was sequenced to enable HybridMelT typing. We found that the presented method is suitable to quickly monitor isolates of ESBL-KLPN; results are obtained in less than 2 hours and at a lower cost than MLST. We identified a local ESBL-KLPN outbreak and a comparison of colonizing and invasive isolates revealed a long term colonization of patients with the same strain.
Department of Clinical Microbiology University Hospital Brno Brno Czech Republic
Department of Internal Medicine Hematology and Oncology Masaryk University Brno Czech Republic
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