BACKGROUND: The use of light emitting diodes (LEDs) brings several key advantages over existing illumination technologies for indoor plant cultivation. Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes. Recent advances allow LEDs to be used with customized wavelengths for plant growth. However, most of these LED growth systems use mixtures of chips emitting in several narrow wavelengths and frequently they are not compatible with existing infrastructures. This study tested the growth of five different plant species under phosphor coated LED-chips fitted into a tube with a standard G13 base that provide continuous visible light illumination with enhanced blue and red light. RESULTS: The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room. Significant differences in heat generation between LEDs and fluorescent tubes were clearly demonstrated. Also, LED lights allowed for better control and stability of preset conditions. Physiological properties such as growth characteristics, biomass, and chlorophyll content were measured and the responses to pathogen assessed for five plant species (both the model plants Arabidopsis thaliana, Nicotiana bentamiana and crop species potato, oilseed rape and soybean) under the different illumination sources. CONCLUSIONS: We showed that polychromatic LEDs provide light of sufficient quality and intensity for plant growth using less than 40% of the electricity required by the standard fluorescent lighting under test. The tested type of LED installation provides a simple upgrade pathway for existing infrastructure for indoor plant growth. Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

Laboratory of Pathological Plant Physiology Institute of Experimental Botany AS CR Rozvojová 313 165 02 Prague 6 Czech Republic

Laboratory of Pathological Plant Physiology Institute of Experimental Botany AS CR Rozvojová 313 165 02 Prague 6 Czech Republic ; Department of Biochemistry and Microbiology University of Chemistry and Technology Prague Technická 5 166 28 Prague 6 Czech Republic

Laboratory of Stress Physiology Institute of Experimental Botany AS CR Rozvojová 313 165 02 Prague 6 Czech Republic

Laboratory of Virology Institute of Experimental Botany AS CR Rozvojová 313 165 02 Prague 6 Czech Republic

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Folta KM, Childers KS. Light as a growth regulator: controlling plant biology with narrow-bandwidth solid-state lighting systems. HortScience Publication Am Soc Horticultural Scie. 2008;43:1957–64.

Folta KM, Maruhnich SA. Green light: a signal to slow down or stop. J Exp Bot. 2007;58:3099–111. doi: 10.1093/jxb/erm130. PubMed DOI

Massa GD, Kim HH, Wheeler RM, Mitchell CA. Plant productivity in response to LED lighting. HortScience Publication Am So Horticultural Sci. 2008;43:1951–6.

Bula RJ, Morrow RC, Tibbitts TW, Barta DJ, Ignatius RW, Martin TS. Light-emitting diodes as a radiation source for plants. HortScience Publication Amn Soc Horticultural Sci. 1991;26:203–5. PubMed

Hoenecke ME, Bula RJ, Tibbitts TW. Importance of ‘blue’ photon levels for lettuce seedlings grown under red-light-emitting diodes. HortScience Publication Am Soc Horticultural Sci. 1992;27:427–30. PubMed

Schwartz A, Zeiger E. Metabolic energy for stomatal opening. Roles of photophosphorylation and oxidative phosphorylation. Planta. 1984;161:129–36. doi: 10.1007/BF00395472. PubMed DOI

Cosgrove DJ, Green PB. Rapid suppression of growth by blue light: BIOPHYSICAL MECHANISM OF ACTION. Plant Physiol. 1981;68:1447–53. doi: 10.1104/pp.68.6.1447. PubMed DOI PMC

Cosgrove DJ. Rapid suppression of growth by blue light: OCCURRENCE, TIME COURSE, AND GENERAL CHARACTERISTICS. Plant Physiol. 1981;67:584–90. doi: 10.1104/pp.67.3.584. PubMed DOI PMC

Blaauw OH, Blaauw-Jansen G, van Leeuwen WJ. An irreversible red-light-induced growth response in Avena. Planta. 1968;82:87–104. doi: 10.1007/BF00384699. PubMed DOI

Barta DJ, Tibbitts TW, Bula RJ, Morrow RC. Evaluation of light emitting diode characteristics for a space-based plant irradiation source. Advances Space Res Official J Committee Space Res. 1992;12:141–9. doi: 10.1016/0273-1177(92)90020-X. PubMed DOI

Croxdale J, Cook M, Tibbitts TW, Brown CS, Wheeler RM. Structure of potato tubers formed during spaceflight. J Exp Bot. 1997;48:2037–43. doi: 10.1093/jxb/48.12.2037. PubMed DOI

Brown CS, Tibbitts TW, Croxdale JG, Wheeler RM. Potato tuber formation in the spaceflight environment. Life Support Biosphere Sci Int J Earth Space. 1997;4:71–6. PubMed

Yano A, Fujiwara K. Plant lighting system with five wavelength-band light-emitting diodes providing photon flux density and mixing ratio control. Plant Methods. 2012;8:46. doi: 10.1186/1746-4811-8-46. PubMed DOI PMC

Moravec T, Schmidt MA, Herman EM, Woodford-Thomas T. Production of Escherichia coli heat labile toxin (LT) B subunit in soybean seed and analysis of its immunogenicity as an oral vaccine. Vaccine. 2007;25:1647–57. doi: 10.1016/j.vaccine.2006.11.010. PubMed DOI

Semenyuk EG, Schmidt MA, Beachy RN, Moravec T, Woodford-Thomas T. Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds. Transgenic Res. 2010;19:987–99. doi: 10.1007/s11248-010-9377-6. PubMed DOI

Navratil O, Bucher P, Vacek J. Transgene coding of a Key enzyme of the Glycolytic pathway helps to decrease sugar content in potato tubers. Czech J Genet Plant. 2012;48:42–5.

Navratil O, Fischer L, Cmejlova J, Linhart M, Vacek J. Decreased amount of reducing sugars in transgenic potato tubers and its influence on yield characteristics. Biol Plantarum. 2007;51:56–60. doi: 10.1007/s10535-007-0011-2. DOI

Plchova H, Moravec T, Dedic P, Cerovska N. Expression of recombinant potato leafroll virus structural and Non-structural proteins for antibody production. J Phytopathol. 2011;159:130–2. doi: 10.1111/j.1439-0434.2010.01740.x. DOI

Sasek V, Novakova M, Jindrichova B, Boka K, Valentova O, Burketova L. Recognition of avirulence gene AvrLm1 from hemibiotrophic ascomycete Leptosphaeria maculans triggers salicylic acid and ethylene signaling in Brassica napus. Molecular Plant-Microbe Interactions MPMI. 2012;25:1238–50. doi: 10.1094/MPMI-02-12-0033-R. PubMed DOI

Novakova M, Sasek V, Dobrev PI, Valentova O, Burketova L. Plant hormones in defense response of Brassica napus to Sclerotinia sclerotiorum - reassessing the role of salicylic acid in the interaction with a necrotroph. Plant Physiol Biochemistry PPB / Societe francaise de physiologie vegetale. 2014;80:308–17. PubMed

Cope KR, Bugbee B. Spectral effects of three types of white light-emitting diodes on plant growth and development: absolute versus relative amounts of blue light. HortScience Publication Am Soc Horticultural Sci. 2013;48:504–9.

Sasek V, Janda M, Delage E, Puyaubert J, Guivarc’h A, Lopez Maseda E, et al. Constitutive salicylic acid accumulation in pi4kIIIbeta1beta2 Arabidopsis plants stunts rosette but not root growth. New phytologist. 2014;203(3):805–16. doi: 10.1111/nph.12822. PubMed DOI

Wildermuth MC, Dewdney J, Wu G, Ausubel FM. Isochorismate synthase is required to synthesize salicylic acid for plant defence. Nature. 2001;414:562–5. doi: 10.1038/35107108. PubMed DOI

Lawton KA, Friedrich L, Hunt M, Weymann K, Delaney T, Kessmann H, et al. Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway. Plant J. 1996;10:71–82. doi: 10.1046/j.1365-313X.1996.10010071.x. PubMed DOI

Folta K, Maruhnich S, Dhingra A, Kumar D. Green light control of plant form and function. Comp Biochem Phys A. 2007;146:S227–8. doi: 10.1016/j.cbpa.2007.01.501. DOI

Nelson JA, Bugbee B. Economic analysis of greenhouse lighting: light emitting diodes vs. high intensity discharge fixtures. PLoS One. 2014;9:e99010. doi: 10.1371/journal.pone.0099010. PubMed DOI PMC

Zhang QY, Wang LY, Kong FY, Deng YS, Li B, Meng QW. Constitutive accumulation of zeaxanthin in tomato alleviates salt stress-induced photoinhibition and photooxidation. Physiol Plantarum. 2012;146:363–73. doi: 10.1111/j.1399-3054.2012.01645.x. PubMed DOI

Hua J. Modulation of plant immunity by light, circadian rhythm, and temperature. Curr Opin Plant Biol. 2013;16:406–13. doi: 10.1016/j.pbi.2013.06.017. PubMed DOI

Olle M, Virsile A. The effects of light-emitting diode lighting on greenhouse plant growth and quality. Agr Food Sci. 2013;22:223–34.

Griebel T, Zeier J. Light regulation and daytime dependency of inducible plant defenses in arabidopsis: Phytochrome signaling controls systemic acquired resistance rather than local defense. Plant Physiol. 2008;147:790–801. doi: 10.1104/pp.108.119503. PubMed DOI PMC

Wang H, Jiang YP, Yu HJ, Xia XJ, Shi K, Zhou YH, et al. Light quality affects incidence of powdery mildew, expression of defence-related genes and associated metabolism in cucumber plants. Eur J Plant Pathol. 2010;127:125–35. doi: 10.1007/s10658-009-9577-1. DOI

Cerovska N, Hoffmeisterova H, Pecenkova T, Moravec T, Synkova H, Plchova H, et al. Transient expression of HPV16 E7 peptide (aa 44–60) and HPV16 L2 peptide (aa 108–120) on chimeric potyvirus-like particles using Potato virus X-based vector. Protein Expr Purif. 2008;58:154–61. doi: 10.1016/j.pep.2007.09.006. PubMed DOI

One-Enzyme RTX-PCR for the Detection of RNA Viruses from Multiple Virus Genera and Crop Plants

Extended Set of GoldenBraid Compatible Vectors for Fast Assembly of Multigenic Constructs and Their Use to Create Geminiviral Expression Vectors