Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
27444706
DOI
10.1016/j.jbiotec.2016.07.013
PII: S0168-1656(16)31420-1
Knihovny.cz E-resources
- Keywords
- 16S rDNA, Bioreactor, Microbiome analysis, Redox potential, Rumen fluid,
- MeSH
- Rumen metabolism microbiology MeSH
- Bacteria classification genetics growth & development isolation & purification MeSH
- Bacteroidetes genetics growth & development isolation & purification MeSH
- Bioreactors microbiology MeSH
- DNA, Bacterial MeSH
- Fermentation MeSH
- Firmicutes genetics growth & development isolation & purification MeSH
- Fatty Acids, Volatile metabolism MeSH
- Microbiota * genetics MeSH
- Oxidation-Reduction MeSH
- Proteobacteria genetics growth & development isolation & purification MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Cattle MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Fatty Acids, Volatile MeSH
- RNA, Ribosomal, 16S MeSH
To study the various processes in the rumen the in vitro techniques are widely used to realize more controlled and reproducible conditions compared to in vivo experiments. Mostly, only the parameters like pH changes, volatile fatty acids content or metabolite production are monitored. In this study we examine the bacterial community dynamics of rumen fluid in course of ten day cultivation realize under standard conditions described in the literature. Whereas the pH values, total VFA content and A/P ratio in bioreactor were consistent with natural conditions in the rumen, the mean redox-potential values of -251 and -243mV were much more negative. For culture-independent assessment of bacterial community composition, the Illumina MiSeq results indicated that the community contained 292 bacterial genera. In course of ten days cultivation a significant changes in the microbial community were measured when Bacteroidetes to Firmicutes ratio changed from 3.2 to 1.2 and phyla Proteobacteria and Actinobacteria represented by genus Bifidobacterium and Olsenella significantly increased. The main responsible factor of these changes seems to be very low redox potential in bioreactor together with accumulation of simple carbohydrates in milieu as a result of limited excretion of fermented feed and absence of nutrient absorbing mechanisms.
References provided by Crossref.org
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