The mvp2 mutation affects the generative transition through the modification of transcriptome pattern, salicylic acid and cytokinin metabolism in Triticum monococcum
Language English Country Germany Media print-electronic
Document type Journal Article
PubMed
27450491
DOI
10.1016/j.jplph.2016.07.005
PII: S0176-1617(16)30125-0
Knihovny.cz E-resources
- Keywords
- Cytokinin, Triticum monococcum, Vegetative/generative transition, Vernalization, mvp2 mutation,
- MeSH
- Biosynthetic Pathways genetics MeSH
- Cytokinins metabolism MeSH
- Gene Deletion MeSH
- Genotype MeSH
- Gene Regulatory Networks MeSH
- Salicylic Acid metabolism MeSH
- RNA, Messenger genetics metabolism MeSH
- Mutation genetics MeSH
- Triticum genetics growth & development MeSH
- Gene Expression Regulation, Plant MeSH
- Reproducibility of Results MeSH
- Plant Proteins genetics metabolism MeSH
- Oligonucleotide Array Sequence Analysis MeSH
- Cluster Analysis MeSH
- Transcriptome genetics MeSH
- Gene Expression Regulation, Developmental MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Cytokinins MeSH
- Salicylic Acid MeSH
- RNA, Messenger MeSH
- Plant Proteins MeSH
Wild type and mvp2 (maintained vegetative phase) deletion mutant T. monococcum plants incapable of flowering were compared in order to determine the effect of the deleted region of chromosome 5A on transcript profile and hormone metabolism. This region contains the vernalization1 (VRN1) gene, a major regulator of the vegetative/generative transition. Transcript profiling in the crowns of T. monococcum during the transition and the subsequent formation of flower primordia showed that 306 genes were affected by the mutation, 198 by the developmental phase and 14 by the interaction of these parameters. In addition, 546 genes were affected by two or three factors. The genes controlled by the deleted region encode transcription factors, antioxidants and enzymes of hormone, carbohydrate and amino acid metabolism. The observed changes in the expression of the gene encoding phenylalanine ammonia lyase (PAL) might indicate the effect of mvp2 mutation on the metabolism of salicylic acid, which was corroborated by the differences in 2-hydroxycinnamic acid and cinnamic acid contents in both of the leaves and crowns, and in the concentrations of salicylic acid and benzoic acid in crowns during the vegetative/generative transition. The amount and ratio of active cytokinins and their derivatives (ribosides, glucosides and phosphates) were affected by developmental changes as well as by mvp2 mutation, too.
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