A comparison of electrochemically pre-treated and spark-platinized carbon fiber microelectrode. Measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine in human urine and plasma
Language English Country Netherlands Media print-electronic
Document type Comparative Study, Journal Article
PubMed
27543016
DOI
10.1016/j.aca.2016.06.044
PII: S0003-2670(16)30807-8
Knihovny.cz E-resources
- Keywords
- 8-oxo-7,8-dihydro-2′-deoxyguanosine, Amperometric detection, Carbon fiber microelectrode, Liquid chromatography, Platinization, Spark discharge,
- MeSH
- 8-Hydroxy-2'-Deoxyguanosine MeSH
- Deoxyguanosine analogs & derivatives blood urine MeSH
- Electrochemical Techniques * MeSH
- Hydrodynamics MeSH
- Carbon Fiber MeSH
- Humans MeSH
- Microelectrodes MeSH
- Carbon chemistry MeSH
- Particle Size MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- 8-Hydroxy-2'-Deoxyguanosine MeSH
- Deoxyguanosine MeSH
- Carbon Fiber MeSH
- Carbon MeSH
A novel method of carbon fiber microelectrode activation using spark discharge was demonstrated and compared to conventional electrochemical pretreatment by potential cycling. The spark discharge was performed at 800 V between the microelectrode connected to positive pole of the power supply and platinum counter electrode. Spark discharge led both to trimming of the fiber tip into conical shape and to the modification of carbon fiber microelectrode with platinum, as proven by scanning electron microscopy and electron dispersive X-ray spectroscopy. After the characterization of electrochemical properties using ferricyanide voltammetry, the activated electrodes were used for electrochemical analysis of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an oxidative stress marker. Subnanomolar detection limits (0.55 nmol L(-1)) in high-performance liquid chromatography were achieved for spark platinized electrodes incorporated into the flow detection cell.
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