A comparison of electrochemically pre-treated and spark-platinized carbon fiber microelectrode. Measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine in human urine and plasma
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články
PubMed
27543016
DOI
10.1016/j.aca.2016.06.044
PII: S0003-2670(16)30807-8
Knihovny.cz E-zdroje
- Klíčová slova
- 8-oxo-7,8-dihydro-2′-deoxyguanosine, Amperometric detection, Carbon fiber microelectrode, Liquid chromatography, Platinization, Spark discharge,
- MeSH
- 8-hydroxy-2'-deoxyguanosin MeSH
- deoxyguanosin analogy a deriváty krev moč MeSH
- elektrochemické techniky * MeSH
- hydrodynamika MeSH
- karbonové vlákno MeSH
- lidé MeSH
- mikroelektrody MeSH
- uhlík chemie MeSH
- velikost částic MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- 8-hydroxy-2'-deoxyguanosin MeSH
- deoxyguanosin MeSH
- karbonové vlákno MeSH
- uhlík MeSH
A novel method of carbon fiber microelectrode activation using spark discharge was demonstrated and compared to conventional electrochemical pretreatment by potential cycling. The spark discharge was performed at 800 V between the microelectrode connected to positive pole of the power supply and platinum counter electrode. Spark discharge led both to trimming of the fiber tip into conical shape and to the modification of carbon fiber microelectrode with platinum, as proven by scanning electron microscopy and electron dispersive X-ray spectroscopy. After the characterization of electrochemical properties using ferricyanide voltammetry, the activated electrodes were used for electrochemical analysis of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an oxidative stress marker. Subnanomolar detection limits (0.55 nmol L(-1)) in high-performance liquid chromatography were achieved for spark platinized electrodes incorporated into the flow detection cell.
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