Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
- Keywords
- ELISA, cerebrospinal fluid, immunoglobulins, isoectric focusing, kappa/lambda ratio,
- MeSH
- Enzyme-Linked Immunosorbent Assay methods MeSH
- Immunoglobulin G cerebrospinal fluid immunology MeSH
- Immunoglobulin kappa-Chains cerebrospinal fluid immunology MeSH
- Immunoglobulin lambda-Chains cerebrospinal fluid immunology MeSH
- Humans MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Immunoglobulin G MeSH
- Immunoglobulin kappa-Chains MeSH
- Immunoglobulin lambda-Chains MeSH
IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using Hevylite™ antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was observed (increased in 16 and decreased in 3 cases). The analysis of light chain composition of intrathecally synthesised immunoglobulins could contribute to our understanding of intrathecal humoral immune response, although its diagnostic utility is limited.
b Clinic of Neurology Fakultni Nemocnice Ostrava Ostrava Czech Republic
c Department of Clinical Biochemistry Institute of Laboratory Medicine Ostrava Czech Republic
Institute of Laboratory Diagnostics Fakultni Nemocnice Ostrava Ostrava Czech Republic
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