Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články
- Klíčová slova
- ELISA, cerebrospinal fluid, immunoglobulins, isoectric focusing, kappa/lambda ratio,
- MeSH
- ELISA metody MeSH
- imunoglobulin G mozkomíšní mok imunologie MeSH
- imunoglobuliny - kappa-řetězce mozkomíšní mok imunologie MeSH
- imunoglobuliny - lambda-řetězce mozkomíšní mok imunologie MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- imunoglobulin G MeSH
- imunoglobuliny - kappa-řetězce MeSH
- imunoglobuliny - lambda-řetězce MeSH
IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using Hevylite™ antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was observed (increased in 16 and decreased in 3 cases). The analysis of light chain composition of intrathecally synthesised immunoglobulins could contribute to our understanding of intrathecal humoral immune response, although its diagnostic utility is limited.
b Clinic of Neurology Fakultni Nemocnice Ostrava Ostrava Czech Republic
c Department of Clinical Biochemistry Institute of Laboratory Medicine Ostrava Czech Republic
Institute of Laboratory Diagnostics Fakultni Nemocnice Ostrava Ostrava Czech Republic
Citace poskytuje Crossref.org
