Interface-free capillary electrophoresis-mass spectrometry system with nanospray ionization-Analysis of dexrazoxane in blood plasma
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27613146
DOI
10.1016/j.chroma.2016.08.042
PII: S0021-9673(16)31119-0
Knihovny.cz E-zdroje
- Klíčová slova
- Capillary electrophoresis, Dexrazoxane, Hydrophobic coating, Interface, Mass spectrometry, Nanospray,
- MeSH
- biochemická analýza krve metody MeSH
- dexrazoxan krev MeSH
- elektroforéza kapilární * MeSH
- ethylendiaminy krev MeSH
- glycin analogy a deriváty krev MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dexrazoxan MeSH
- ethylendiaminy MeSH
- glycin MeSH
- ICRF 198 MeSH Prohlížeč
The newly developed interface-free capillary electrophoresis-nanospray/mass spectrometry system (CE-nESI/MS) was applied for rapid analysis of the cardioprotective drug dexrazoxane and its hydrolysed form ADR-925 in deproteinized blood plasma samples. The aim of this study was to test the simplest possible CE-nESI/MS instrumentation for analyses of real samples. This interface-free system, utilizing single piece of a narrow bore capillary as both the electrophoretic separation column and the nanospray emitter, was operated at a flow rate of 30nL/min. Excellent electrophoretic separation and sensitive nanospray ionization was achieved with the use of only one high voltage power supply. In addition, hydrophobic external coating was developed and tested for additional stability of the nanospray ionization. To our knowledge this is the first study devoted to the analysis of dexrazoxane and ADR-925 by capillary electrophoresis-mass spectrometry.
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