Interface-free capillary electrophoresis-mass spectrometry system with nanospray ionization-Analysis of dexrazoxane in blood plasma
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
27613146
DOI
10.1016/j.chroma.2016.08.042
PII: S0021-9673(16)31119-0
Knihovny.cz E-resources
- Keywords
- Capillary electrophoresis, Dexrazoxane, Hydrophobic coating, Interface, Mass spectrometry, Nanospray,
- MeSH
- Blood Chemical Analysis methods MeSH
- Dexrazoxane blood MeSH
- Electrophoresis, Capillary * MeSH
- Ethylenediamines blood MeSH
- Glycine analogs & derivatives blood MeSH
- Spectrometry, Mass, Electrospray Ionization * MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Dexrazoxane MeSH
- Ethylenediamines MeSH
- Glycine MeSH
- ICRF 198 MeSH Browser
The newly developed interface-free capillary electrophoresis-nanospray/mass spectrometry system (CE-nESI/MS) was applied for rapid analysis of the cardioprotective drug dexrazoxane and its hydrolysed form ADR-925 in deproteinized blood plasma samples. The aim of this study was to test the simplest possible CE-nESI/MS instrumentation for analyses of real samples. This interface-free system, utilizing single piece of a narrow bore capillary as both the electrophoretic separation column and the nanospray emitter, was operated at a flow rate of 30nL/min. Excellent electrophoretic separation and sensitive nanospray ionization was achieved with the use of only one high voltage power supply. In addition, hydrophobic external coating was developed and tested for additional stability of the nanospray ionization. To our knowledge this is the first study devoted to the analysis of dexrazoxane and ADR-925 by capillary electrophoresis-mass spectrometry.
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