Mycophagy should not be considered as a single and homogeneous category of nutritional biology due to the specific symbiotic chitinolytic bacteria associated with mites and fungi. To test interaction among mites, fungi, and chitinolytic bacteria, experiments were conducted on the model species Tyrophagus putrescentiae (Schrank). Mucor sp, Alternaria alternata, Penicillium claviforme, P. griseofulvum, and Verticillium sp. were plated onto malt agar and offered to T. putrescentiae in the laboratory. Mites were evaluated utilizing microanatomical examination based on histology, excrement analysis using fluorescence microscopy, bacterial plating, impact of mite homogenate on fungi in Petri dishes, reproduction of mites feeding upon each fungus, and isolation of associated bacteria inside mites. There were clear differences regarding the digested spores of different fungi passing through the gut and subsequently in the feces. Abundances of bacterial cells in excrement also corresponded to the fungi offered. The extracts from mites had chitinolytic activity, and the plated bacteria are known to produce exochitinases. The various feeding patterns observed were caused by differences in the cell wall structures of the tested fungi. The study illustrates that mycophagy in saprophagous mites does not consist of a single pattern, but rather that it can be classified into several sub-patterns depending upon the digested fungal species and its parts. The results point to a nearly symbiotic relationship between chitinolytic bacteria and digested fungi in mycophagous microarthropods.

Crop Research Institute Drnovská 507 73 Prague 6 Ruzyně 161 06 Czech Republic

Department of Zoology Faculty of Science Charles University Viničná 7 Prague 2 CZ 128 44 Czech Republic

Zobrazit více v PubMed

Aktuganov G. E., Melent'ev A. I., Kuz'mina L. Y., Galimzyanova N. F., Shirokov A. V. 2004. The chitinolytic activity of PubMed

Anderson J. M. 1975. The enigma of soil animal species diversity, pp. 517

Brurberg M. B., Synstad B., Klemsdal S. S., van Aalten D. M. F., Sundheim L., Eijsink G. H. 2001. Chitinases from

Chang W. T., Chen C. S., Wang S. L. 2003. An antifungal chitinase produced by PubMed

Citterio B., Malatesta M., Battistelli S., Marcheggiani F., Baffone W., Saltarelli R., Stocchi V., Gazzanelli G. 2001. Possible involvement of PubMed

Czajkowska B. 1970. Rozwój rozkruszków na niektórych gatunkach grzybów. Zesz. Problem. Postep.Nauk Roln. 109: 219–227.

Erban T., Hubert J. 2008. Digestive function of lysozyme in synanthropic acaridid mites enables utilization of bacteria as a food source. Exp. Appl. Acarol. 44: 199–212. PubMed

Haq M. A. 1981. Feeding habits of ten species of

Hubert J., Nesvorná M., Ságová-Marečková M., Kopecký J. 2012. Shift of bacterial community in synanthropic mite PubMed PMC

Hudson H. J. 1986. Fungal biology. Edward Arnold, London.

Jaspers E., Overmann J. 2004. Ecological significance of microdiversity: identical 16S rRNA gene sequence can be found in bacteria with highly divergent genomes and ecophysiologies. Appl. Environ. Microbiol. 70: 4831–4839. PubMed PMC

Jollès P., Muzzarelli R.A.A. (eds.). 1999. Chitin and chitinases. Birkhäuser, Basel.

Kaneko N. 1988. Feeding habits and cheliceral size of

Kishore G. K., Pande S., Podile A. R. 2005. Chitin-supplemented foliar application of

Kobayashi D. Y., Reedy R. M., Bick J. A., Oudemans P. V. 2002. Characterization of chitinase gene from PubMed PMC

Koukol O., Mourek J., Janovský Z., Černá K. 2009. Do

Luxton M. 1972. Studies on the

Maraun M., Erdmann G., Fischer B. M., Pollierer M. M., Norton R. A., Schneider K., Scheu S. 2011. Stable isotopes revisited: their use and limits for

Maraun M., Martens H., Migge M., Theenhaus A., Scheu S. 2003. Adding to the ‘enigma of soil animal diversity’: fungal feeders and saprophagous soil invertebrates prefer similar food substrates. Eur. J. Soil Biol. 39: 85–95.

Merzendorfer H., Zimoch L. 2003. Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases. J. Exp. Biol. 206: 4393–4412. PubMed

Pankiewicz-Nowicka D., Boczek J., Davis R. 1984. Food selection in

Ruess L., Häggblomd M. M., Zapata E. J. G., Dighton J. 2002. Fatty acids of fungi and nematodes—possible biomarkers in the soil food chain? Soil Biol. Biochem. 34: 745–756.

Schneider K., Maraun M. 2005. Feeding preferences among dark pigmented fungi (“Dematiacea”) indicate trophic niche differentiation of

Schneider K., Migge S., Norton R. A., Scheu S., Langel R., Reineking A., Maraun M. 2004a. Trophic niche differentiation in soil microarthropods (Oribatida, Acari): evidence from stable isotope ratios (

Schneider K., Renker C., Maraun M. 2005. PubMed

Schneider K., Renker C., Scheu S., Maraun M. 2004b. Feeding biology of

Schuster R. 1956. Der Anteil der Oribatiden an den Zersetzungsvorgängen im Boden. Z. Morphol. Ökol. Tiere. 45: 1–33.

Siepel H., de Ruiter-Dijkman E. M. 1993. Feeding guilds of

Smrž J. 1989. Internal anatomy of PubMed

Smrž J., Trelova M. 1995. The association of bacteria and some soil mites (Acari: Oribatida and Acaridida). Acta Zool.Fenn. 196: 120–123.

Smrž J. 2000. A modified test for chitinase and cellulase activity in soil mites. Pedobiologia. 44: 186–189.

Smrž J. 2002a. Nutritional biology: the basic step in the autecological studies (multi-methodical approach). Eur. J. Soil Biol. 38: 35–38.

Smrž J. 2002b. The excrement analysis—the useful tool for the biological and autecological studies in soil zoology, pp. 185–189.

Smrž J. 2003. Microanatomical and biological aspects of bacterial associations in PubMed

Smrž J. 2009. Mycophagy—dream and reality in soil saprophagous mites (Acari: Oribatida and Acaridida), pp 147–150.

Smrž J., Čatská V. 1987. Food selection of the field population of

Smrž J., Čatská V. 1989. The effect of the consumption of some soil fungi on the internal microanatomy of the mite

Smrž J., Čatská V. 2010. Mycophagous mites and their internal associated bacteria cooperate to digest chitin in soil. Symbiosis. 52: 33–40.

Smrž J., Jungová E. 1989. The ecology of a field population of

Smrž J., Norton R. A. 2004. Food selection and internal processing in

Smrž J., Soukalová H. 2008. Mycophagous mites (Acari: Oribatida and Acaridida) and their cooperation with chitinolytic bacteria, pp. 374–377.

Smrž J., Svobodová J., Čatská V. 1991. Synergetic participation

Wallwork J. A. 1976. The distribution and diversity of soil fauna. Academic Press, London.

Zhang Z., Yuen G., Sarath Y. G., Penheiter A. R. 2001. Chitinases from the plant disease biocontrol agent. Stenotrophomonas Maltophilia C3. Phytopathol. 91: 204–211. PubMed

Zinkler D. 1972. Vergleichende Untersuchungen zum Wirkungsspektrum der Carbohydrases laubstreubewohnender Oribatiden. Verh. Deutsch. Zool. Gesell. 65: 149–153.

Mycophagy: A Global Review of Interactions between Invertebrates and Fungi

Two Populations of Mites (Tyrophagus putrescentiae) Differ in Response to Feeding on Feces-Containing Diets

Experimental Manipulation Shows a Greater Influence of Population than Dietary Perturbation on the Microbiome of Tyrophagus putrescentiae