High Levels of Soluble Endoglin Induce a Proinflammatory and Oxidative-Stress Phenotype Associated with Preserved NO-Dependent Vasodilatation in Aortas from Mice Fed a High-Fat Diet
Language English Country Switzerland Media print-electronic
Document type Journal Article
PubMed
27721318
DOI
10.1159/000448996
PII: 000448996
Knihovny.cz E-resources
- MeSH
- Aorta drug effects metabolism physiopathology MeSH
- Atherosclerosis blood genetics metabolism physiopathology MeSH
- Biomarkers metabolism MeSH
- Diet, High-Fat * MeSH
- Endoglin blood genetics metabolism MeSH
- Phenotype MeSH
- Adaptation, Physiological MeSH
- Genetic Predisposition to Disease MeSH
- Humans MeSH
- Inflammation Mediators metabolism MeSH
- Disease Models, Animal MeSH
- Mice, Inbred C57BL MeSH
- Mice, Inbred CBA MeSH
- Mice, Transgenic MeSH
- Aortic Diseases blood genetics metabolism physiopathology MeSH
- Nitric Oxide metabolism MeSH
- Oxidative Stress * MeSH
- Up-Regulation MeSH
- Vasodilation * drug effects MeSH
- Vasodilator Agents pharmacology MeSH
- Dose-Response Relationship, Drug MeSH
- Inflammation blood genetics metabolism physiopathology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Biomarkers MeSH
- Endoglin MeSH
- ENG protein, human MeSH Browser
- Inflammation Mediators MeSH
- Nitric Oxide MeSH
- Vasodilator Agents MeSH
AIMS: A soluble form of endoglin (sEng) was proposed to participate in the induction of endothelial dysfunction in small blood vessels. Here, we tested the hypothesis that high levels of sEng combined with a high-fat diet induce endothelial dysfunction in an atherosclerosis-prone aorta. METHODS AND RESULTS: Six-month-old female and male transgenic mice overexpressing human sEng (Sol-Eng+) with low (Sol-Eng+low) or high (Sol-Eng+high) levels of plasma sEng were fed a high-fat rodent diet containing 1.25% cholesterol and 40% fat for 3 months. The plasma cholesterol and mouse sEng levels did not differ in the Sol-Eng+high and Sol-Eng+low mice. The expression of proinflammatory (P-selectin, ICAM-1, pNFκB and COX-2) and oxidative-stress-related markers (HO-1, NOX-1 and NOX-2) in the aortas of Sol-Eng+high female mice was significantly higher than in Sol-Eng+low female mice. Endothelium-dependent vasodilatation induced by acetylcholine was preserved better in the Sol-Eng+ high female mice than in the Sol-Eng+low female mice. CONCLUSION: These results suggest that high concentrations of sEng in plasma in combination with a high-fat diet induce the simultaneous activation of proinflammatory, pro-oxidative and vasoprotective mechanisms in mice aorta and the balance of these biological processes determines whether the final endothelial phenotype is adaptive or maladaptive.
References provided by Crossref.org
Kinetics of Biomarkers of Oxidative Stress in Septic Shock: A Pilot Study
High Soluble Endoglin Levels Affect Aortic Vascular Function during Mice Aging
Long term effects of soluble endoglin and mild hypercholesterolemia in mice hearts
High soluble endoglin levels do not induce changes in structural parameters of mouse heart
