• Klíčová slova
• n-3 PUFA,
• MeSH
• COVID-19 * komplikace   patofyziologie   prevence a kontrola   MeSH
• kritický stav mortalita   MeSH
• lidé MeSH
• nenasycené mastné kyseliny * farmakologie   terapeutické užití   MeSH
• syndrom uvolnění cytokinů komplikace   prevence a kontrola   MeSH
• výživové doporučené dávky MeSH
• zánět krev   patofyziologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• přehledy MeSH

This study aimed to investigate the effects of a single bench press (BP) vs. leg press (LP) resistance training sessions on testosterone, cortisol, C-reactive protein (CRP) interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) concentrations, and creatine kinase (CK) activity in strength-trained males. Eleven strength-trained males participated in a cross-over randomized trial, undergoing two experimental sessions each consisting of five sets of the BP or the LP exercise to volitional failure with a load corresponding to 50% of one-repetition maximum. Blood samples were taken at baseline (BA), immediately post (POST), and 1 h after the cessation of exercise (POST-1). A significant increase in IL-6 concentration from BA to POST-1 was observed during the LP condition (p = 0.004; effect size [ES] = 0.64). Additionally, a significant main effect of time was found for increasing testosterone concentrations from BA to POST exercise (p = 0.014; ES = 0.25). A significantly lower cortisol concentration at POST-1 compared to POST (p = 0.001; ES = 1.02) was noted in the BP condition. Furthermore, a significantly lower cortisol concentration was found at POST-1 in the BP compared to the LP condition (p = 0.022; ES = 1.3). A significant increase in CK activity was reported from BA to POST (p = 0.024; ES = 0.69) and POST-1 (p = 0.045; ES = 0.55) during the LP condition, and from BA to POST-1 (p = 0.014; ES = 0.96) during the BP condition. No significant differences were found in the CRP (p = 0.659) and TNF-α concentrations (p = 0.487). These results suggest that the amount of muscle mass engaged during the resistance exercise may influence the changes in IL-6 and cortisol concentrations. Larger muscle groups, as engaged in the LP, more likely lead to elevated concentrations of IL-6 myokine.

• MeSH
• C-reaktivní protein metabolismus   MeSH
• dospělí MeSH
• hydrokortison * krev   MeSH
• interleukin-6 * krev   MeSH
• klinické křížové studie MeSH
• kreatinkinasa krev   MeSH
• lidé MeSH
• mladý dospělý MeSH
• odporový trénink * MeSH
• testosteron * krev   MeSH
• TNF-alfa * krev   MeSH
• zánět krev   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• randomizované kontrolované studie MeSH

The precise link between inflammation and pathogenesis of myelodysplastic syndrome (MDS) is yet to be fully established. We developed a novel method to measure ASC/NLRP3 protein specks which are specific for the NLRP3 inflammasome only. We combined this with cytokine profiling to characterise various inflammatory markers in a large cohort of patients with lower risk MDS in comparison to healthy controls and patients with defined autoinflammatory disorders (AIDs). The ASC/NLRP3 specks were significantly elevated in MDS patients compared to healthy controls (p < 0.001) and these levels were comparable to those found in patients with AIDs. The distribution of protein specks positive only for ASC was different to ASC/NLRP3 ones suggesting that other ASC-containing inflammasome complexes might be important in the pathogenesis of MDS. Patients with MDS-SLD had the lowest levels of interleukin (IL)-1β, tumour necrosis factor (TNF), IL-23, IL-33, interferon (IFN) γ and IFN-α2, compared to other diagnostic categories. We also found that inflammatory cytokine TNF was positively associated with MDS progression to a more aggressive form of disease and IL-6 and IL-1β with time to first red blood cell transfusion. Our study shows that there is value in analysing inflammatory biomarkers in MDS, but their diagnostic and prognostic utility is yet to be fully validated.

• MeSH
• biologické markery krev   MeSH
• cytokiny * krev   MeSH
• dospělí MeSH
• inflamasomy MeSH
• lidé středního věku MeSH
• lidé MeSH
• myelodysplastické syndromy * krev   MeSH
• protein NLRP3 krev   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• studie případů a kontrol MeSH
• zánět krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Práce byla podpořena grantem MZ ČR – RVO (FNOl, 00098892). Vitamin D je významným steroidním hormonálním prekurzorem s vlivem jak na kostní metabolismus, tak i na imunitní děje a další orgánové systémy. Pacienti, kterým je podávána enterální nebo parenterální výživa, patří mezi rizikové stran možného rozvoje deficitu vitaminu D. Článek se věnuje analýze skupin pacientů v riziku deplece vitaminu D z pohledu nutricionisty a uvádí dostupné doporučené postupy pro monitoraci hladin a suplementaci vitaminu D u pacientů na nutriční podpoře.

Vitamin D is an important steroid hormone precursor with an effect on bone metabolism, immune processes and other organ systems. Patients receiving enteral or parenteral nutrition are at risk of developing vitamin D deficiency. The article provides an analysis of group of patients at risk of vitamin D deficiency form the point of view of a nutritionists and present available recommendations for monitoring and supplementation of vitamin D in patients on nutritional support.

• MeSH
• lidé MeSH
• mikroživiny aplikace a dávkování   krev   MeSH
• nedostatek vitaminu D etiologie   krev   terapie   MeSH
• nutriční podpora * škodlivé účinky   MeSH
• poruchy výživy etiologie   krev   patologie   terapie   MeSH
• rizikové faktory MeSH
• vitamin D * aplikace a dávkování   krev   MeSH
• zánět krev   patofyziologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH
• přehledy MeSH

OBJECTIVES: Concentrations of neopterin, kynurenine and kynurenine/tryptophan ratios predict prognosis and the need for oxygen therapy in patients hospitalized for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The aims of the present study were to evaluate the changes of these biomarkers early in the course of infection, the association with the prior coronavirus disease (COVID-19) vaccination and therapeutic administration of Anti-SARS-CoV-2 monoclonal antibodies, investigation of other potential biomarkers including neuropilin, 8-hydroxy-2-deoxyguanosine and 8-hydroxyguanosine in patients hospitalized with SARS-CoV-2 infection and an assessment of these biomarkers and vitamins A, E and D in patients with post-COVID syndrome. METHODS: Urine and blood samples were obtained on the 1st to the 4th day and 4th to 7th day from 108 patients hospitalized with COVID-19. Chromatography tandem mass spectrometry methods were used to analyse neopterin, kynurenine, tryptophan, liposoluble vitamins, and DNA damage biomarkers. RESULTS: A statistically significant decrease of neopterin, kynurenine and kynurenine/tryptophan ratios was observed on after 4th to 7th day of hospitalization, and concentrations of these biomarkers were increased in patients with poor prognosis and subsequent post-COVID syndrome. The concentrations of remaining biomarker and vitamins were not associated with outcomes, although markedly decreased concentrations of vitamin A, E and D were noted. CONCLUSIONS: The concentrations of neopterin, kynurenine and kynurenine/tryptophan ratios decrease during the course of infection SARS-CoV-2 and are associated with the post-COVID syndrome. No other prognostic biomarkers were identified.

• MeSH
• biologické markery * krev   MeSH
• COVID-19 * krev   MeSH
• dospělí MeSH
• hospitalizace MeSH
• kynurenin * krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• neopterin * krev   moč   MeSH
• postakutní syndrom COVID-19 MeSH
• SARS-CoV-2 * izolace a purifikace   MeSH
• senioři MeSH
• tryptofan * krev   MeSH
• vitamin A krev   MeSH
• vitamin D krev   MeSH
• vitamin E krev   MeSH
• vitaminy krev   MeSH
• zánět krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

• Klíčová slova
• hsCRP,
• MeSH
• ateroskleróza krev   metabolismus   patofyziologie   prevence a kontrola   MeSH
• C-reaktivní protein * analýza   MeSH
• kardiovaskulární nemoci klasifikace   krev   MeSH
• lidé MeSH
• rizikové faktory kardiovaskulárních chorob MeSH
• zánět krev   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• přehledy MeSH

Cíl: Naším cílem bylo zjistit, zda sérový delta index neutrofilů a další parametry systémového zánětlivého indexu mohou mít při použití s jinými bio chemickými markery u preeklampsie a HELLP syndromu pomocný účinek v diagnostice a určit roli zánětu v patogenezi těchto onemocnění. Materiál a metody: Do studie bylo zahrnuto 121 těhotných žen, které splnily kritéria pro zařazení a vyloučení. Do sledované skupiny bylo zařazeno 52 těhotných s diagnostikovanou preeklampsií, 19 těhotných s diagnostikovaným HELLP syndromem a do kontrolní skupiny 50 zdravých těhotných žen. Demografické údaje, hematologické a bio chemické parametry a zánětlivé markery (sérový neutrofilní delta index – DNI – a systémové parametry zánětlivého indexu) skupin byly zaznamenány a porovnány mezi skupinami. Výsledky: Z hlediska poměru neutrofilních lymfocytů, poměru lymfocytů trombocytů a DNI byla skupina HELLP odlišná od obou skupin. Kontrolní skupina a skupina s preeklampsií byly podobné. Z hlediska poměru monocytů k lymfocytům byla skupina preeklampsie odlišná od obou skupin. Kontrolní a HELLP skupiny byly podobné. Z hlediska indexu systémového zánětu byly všechny skupiny podobné. Závěr: V naší studii jsme zjistili, že využití DNI v mateřském séru spolu s dalšími bio chemickými parametry může pomoci v diagnostice preeklampsie a HELLP syndromu a zánět může hrát roli v patogenezi těchto onemocnění.

Objective: We aimed to determine whether the serum delta neutrophil index and other systemic inflammatory index parameters can have an auxiliary effect in the diagnosis when used with other bio chemical markers in preeclampsia and HELLP syndrome and to determine the role of inflammation in the pathogenesis of these diseases. Materials and methods: 121 pregnant women who met the inclusion and exclusion criteria were included in the study. 52 pregnant women diagnosed with preeclampsia and 19 pregnant women diagnosed with HELLP syndrome were included in the study group, and 50 healthy pregnant women were included in the control group. Demographic data, hematological and bio chemical parameters, and inflammatory markers (serum delta neutrophil index – DNI – and systemic inflammatory index parameters) of the groups were recorded and compared between groups. Results: In terms of neutrophil lymphocyte ratio, platelet lymphocyte ratio, and DNI, the HELLP group was different from both groups. The control and preeclampsia groups were similar. In terms of monocyte-to-lymphocyte ratio, the preeclampsia group was different from both groups. The control and HELLP groups were similar. In terms of the systemic inflammatory index, all groups were similar. Conclusion: In our study, we found that when maternal serum DNI values are used together with other bio chemical parameters, it can help in the diagnosis of preeclampsia and HELLP syndrome, and inflammation may play a role in the pathogenesis of these diseases.

• Klíčová slova
• delta neutrophil index, systemic inflammation index,
• MeSH
• biologické markery * krev   MeSH
• dospělí MeSH
• HELLP syndrom diagnóza   MeSH
• lidé MeSH
• neutrofily MeSH
• preeklampsie diagnóza   MeSH
• statistika jako téma MeSH
• studie případů a kontrol MeSH
• těhotenství MeSH
• zánět krev   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH

Compelling evidence supports the health benefits of physical exercise on the immune system, possibly through the molecules secreted by the skeletal muscles known as myokines. Herein, we assessed the impact of exercise interventions on plasma Heat shock protein 90 (Hsp90) levels in 27 patients with idiopathic inflammatory myopathies (IIM) compared with 23 IIM patients treated with standard-of-care immunosuppressive therapy only, and in 18 healthy subjects undergoing strenuous eccentric exercise, and their associations with the traditional serum markers of muscle damage and inflammation. In contrast to IIM patients treated with pharmacotherapy only, in whom we demonstrated a significant decrease in Hsp90 over 24 weeks, the 24-week exercise program resulted in a stabilization of Hsp90 levels. These changes in Hsp90 levels were associated with changes in several inflammatory cytokines/chemokines involved in the pathogenesis of IIM or muscle regeneration in general. Strenuous eccentric exercise in healthy volunteers induced a brief increase in Hsp90 levels with a subsequent return to baseline levels at 14 days after the exercise, with less pronounced correlations to systemic inflammation. In this study, we identified Hsp90 as a potential myokine and mediator for exercise-induced immune response and as a potential biomarker predicting improvement after physiotherapy in muscle endurance in IIM.

• MeSH
• biologické markery krev   metabolismus   MeSH
• chemokiny krev   metabolismus   MeSH
• cytokiny krev   metabolismus   MeSH
• imunosupresiva terapeutické užití   MeSH
• kosterní svaly * metabolismus   MeSH
• lidé MeSH
• myozitida * krev   farmakoterapie   metabolismus   terapie   MeSH
• proteiny tepelného šoku HSP90 * krev   metabolismus   MeSH
• terapie cvičením * MeSH
• zánět * krev   farmakoterapie   metabolismus   terapie   MeSH
• zdraví dobrovolníci pro lékařské studie MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH

Familial hypercholesterolemia (FH), is an autosomal dominant disorder caused by mutations in the LDLR, APOB, PCSK9, and APOE genes and is characterized by high plasma levels of total and low-density lipoprotein (LDL) cholesterol. Our study aimed to analyze the influences of two different therapies on a wide spectrum of plasma protein biomarkers of cardiovascular diseases. Plasma from FH patients under hypolipidemic therapy (N = 18; men = 8, age 55.4 ± 13.1 years) and patients under combined long-term LDL apheresis/hypolipidemic therapy (N = 14; men = 7; age 58.0 ± 13.6 years) were analyzed in our study. We measured a profile of 184 cardiovascular disease (CVD) associated proteins using a proximity extension assay (PEA). Hypolipidemic therapy significantly (all p < 0.01) influenced 10 plasma proteins (TM, DKK1, CCL3, CD4, PDGF subunit B, AGRP, IL18, THPO, and LOX1 decreased; ST2 increased). Under combined apheresis/hypolipidemic treatment, 18 plasma proteins (LDLR, PCSK9, MMP-3, GDF2, CTRC, SORT1, VEGFD, IL27, CCL24, and KIM1 decreased; OPN, COL1A1, KLK6, IL4RA, PLC, TNFR1, GLO1, and PTX3 increased) were significantly affected (all p < 0.006). Hypolipidemic treatment mainly affected biomarkers involved in vascular endothelial maintenance. Combined therapy influenced proteins that participate in cholesterol metabolism and inflammation.

• MeSH
• anticholesteremika terapeutické užití   MeSH
• biologické markery krev   MeSH
• cholesterol krev   metabolismus   MeSH
• dospělí MeSH
• hyperlipoproteinemie typ II krev   farmakoterapie   genetika   patologie   MeSH
• kardiovaskulární nemoci krev   farmakoterapie   genetika   patologie   MeSH
• krevní proteiny klasifikace   genetika   izolace a purifikace   MeSH
• lidé středního věku MeSH
• lidé MeSH
• senioři MeSH
• zánět krev   genetika   metabolismus   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

PURPOSE: To examine the predictive and prognostic value of preoperative Systemic Immune-inflammation Index (SII) in patients with radio-recurrent prostate cancer (PCa) treated with salvage radical prostatectomy (SRP). MATERIALS AND METHODS: This multicenter retrospective study included 214 patients with radio-recurrent PCa, treated with SRP between 2007 and 2015. SII was measured preoperatively (neutrophils × platelets/lymphocytes) and the cohort was stratified using optimal cut-off. Uni- and multivariable logistic and Cox regression analyses were performed to evaluate the predictive and prognostic value of SII as a preoperative biomarker. RESULTS: A total of 81 patients had high preoperative SII (≥ 730). On multivariable logistic regression modeling, high SII was predictive for lymph node metastases (OR 3.32, 95% CI 1.45-7.90, p = 0.005), and non-organ confined disease (OR 2.55, 95% CI 1.33-4.97, p = 0.005). In preoperative regression analysis, high preoperative SII was an independent prognostic factor for cancer-specific survival (CSS; HR 10.7, 95% CI 1.12-103, p = 0.039) and overall survival (OS; HR 8.57, 95% CI 2.70-27.2, p < 0.001). Similarly, in postoperative multivariable models, SII was associated with worse CSS (HR 22.11, 95% CI 1.23-398.12, p = 0.036) and OS (HR 5.98, 95% CI 1.67-21.44, p = 0.006). Notably, the addition of SII to preoperative reference models improved the C-index for the prognosis of CSS (89.5 vs. 80.5) and OS (85.1 vs 77.1). CONCLUSIONS: In radio-recurrent PCa patients, high SII was associated with adverse pathological features at SRP and survival after SRP. Preoperative SII could help identify patients who might benefit from novel imaging modalities, multimodal therapy or a closer posttreatment surveillance.

• MeSH
• brachyterapie MeSH
• lidé středního věku MeSH
• lidé MeSH
• lokální recidiva nádoru imunologie   mortalita   patologie   chirurgie   MeSH
• lymfatické uzliny patologie   MeSH
• lymfocyty * MeSH
• míra přežití MeSH
• multivariační analýza MeSH
• nádory prostaty imunologie   mortalita   patologie   chirurgie   MeSH
• neutrofily * MeSH
• počet leukocytů MeSH
• počet lymfocytů MeSH
• počet trombocytů MeSH
• předoperační období MeSH
• prognóza MeSH
• proporcionální rizikové modely MeSH
• prostatektomie * MeSH
• radioterapie s modulovanou intenzitou MeSH
• retrospektivní studie MeSH
• senioři MeSH
• staging nádorů MeSH
• trombocyty * MeSH
• záchranná terapie * MeSH
• zánět krev   imunologie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH