Luminescent Ruthenium(II) Polypyridyl Complexes as Nonviral Carriers for DNA Delivery
Language English Country Germany Media print-electronic
Document type Journal Article
- Keywords
- DNA, electrochemistry, luminescence, photochemistry, ruthenium,
- MeSH
- Circular Dichroism MeSH
- DNA administration & dosage chemistry MeSH
- Genetic Vectors chemistry MeSH
- HeLa Cells MeSH
- Coordination Complexes chemical synthesis chemistry MeSH
- Crystallography, X-Ray MeSH
- Quantum Theory * MeSH
- Humans MeSH
- Luminescence MeSH
- Microscopy, Atomic Force MeSH
- Molecular Structure MeSH
- Pyridines chemistry MeSH
- Ruthenium chemistry MeSH
- Gene Transfer Techniques instrumentation MeSH
- Cell Survival MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA MeSH
- Coordination Complexes MeSH
- Pyridines MeSH
- Ruthenium MeSH
Two new luminescent ruthenium(II) polypyridyl complexes, [Ru(bpy)2 (tpt-phen)]Cl2 (1; bpy=2,2'-bipyridine, tpt-phen=triptycenyl-1,10-phenanthroline) and [Ru(phen)2 (tpt-phen)]Cl2 (2; phen=1,10-phenanthroline), have been developed as potential nonviral vectors for DNA delivery. Photophysical and electrochemical properties of the complexes have been investigated and corroborated with electronic structure calculations. DNA condensation by these complexes has been investigated by UV/Vis and emission spectroscopy, circular dichroism spectroscopy, atomic force microscopy, dynamic light scattering, confocal microscopy, and electrophoretic mobility studies. These complexes interact with DNA and efficiently condense DNA into globular nanoparticles that are taken up efficiently by HeLa cells. DNA cleavage inability and biocompatibility of complexes have been explored. Both complexes have good gene transfection abilities.
Central European Institute of Technology Masaryk University Kamenice 5 62500 Brno Czech Republic
Department of Chemistry Karnatak University Dharwad 580003 Karnataka India
Department of Chemistry Masaryk University Kotlarska 2 61137 Brno Czech Republic
Department of Chemistry Savitribhai Phule University of Pune Pune 411007 Maharashtra India
References provided by Crossref.org
Metallohelix vectors for efficient gene delivery via cationic DNA nanoparticles
