Luminescent Ruthenium(II) Polypyridyl Complexes as Nonviral Carriers for DNA Delivery
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27860328
DOI
10.1002/asia.201601515
Knihovny.cz E-zdroje
- Klíčová slova
- DNA, electrochemistry, luminescence, photochemistry, ruthenium,
- MeSH
- cirkulární dichroismus MeSH
- DNA aplikace a dávkování chemie MeSH
- genetické vektory chemie MeSH
- HeLa buňky MeSH
- komplexní sloučeniny chemická syntéza chemie MeSH
- krystalografie rentgenová MeSH
- kvantová teorie * MeSH
- lidé MeSH
- luminiscence MeSH
- mikroskopie atomárních sil MeSH
- molekulární struktura MeSH
- pyridiny chemie MeSH
- ruthenium chemie MeSH
- technika přenosu genů přístrojové vybavení MeSH
- viabilita buněk MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA MeSH
- komplexní sloučeniny MeSH
- pyridiny MeSH
- ruthenium MeSH
Two new luminescent ruthenium(II) polypyridyl complexes, [Ru(bpy)2 (tpt-phen)]Cl2 (1; bpy=2,2'-bipyridine, tpt-phen=triptycenyl-1,10-phenanthroline) and [Ru(phen)2 (tpt-phen)]Cl2 (2; phen=1,10-phenanthroline), have been developed as potential nonviral vectors for DNA delivery. Photophysical and electrochemical properties of the complexes have been investigated and corroborated with electronic structure calculations. DNA condensation by these complexes has been investigated by UV/Vis and emission spectroscopy, circular dichroism spectroscopy, atomic force microscopy, dynamic light scattering, confocal microscopy, and electrophoretic mobility studies. These complexes interact with DNA and efficiently condense DNA into globular nanoparticles that are taken up efficiently by HeLa cells. DNA cleavage inability and biocompatibility of complexes have been explored. Both complexes have good gene transfection abilities.
Central European Institute of Technology Masaryk University Kamenice 5 62500 Brno Czech Republic
Department of Chemistry Karnatak University Dharwad 580003 Karnataka India
Department of Chemistry Masaryk University Kotlarska 2 61137 Brno Czech Republic
Department of Chemistry Savitribhai Phule University of Pune Pune 411007 Maharashtra India
Citace poskytuje Crossref.org
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