An intra-laboratory cultural and real-time PCR method comparison and evaluation for the detection of subclinical paratuberculosis in dairy herds
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu srovnávací studie, hodnotící studie, časopisecké články
PubMed
27988836
DOI
10.1007/s12223-016-0488-1
PII: 10.1007/s12223-016-0488-1
Knihovny.cz E-zdroje
- MeSH
- asymptomatické infekce * MeSH
- bakteriologické techniky metody MeSH
- diagnostické techniky molekulární metody MeSH
- feces mikrobiologie MeSH
- kvantitativní polymerázová řetězová reakce metody MeSH
- Mycobacterium avium subsp. paratuberculosis klasifikace genetika růst a vývoj izolace a purifikace MeSH
- nemoci skotu diagnóza MeSH
- odběr biologického vzorku metody MeSH
- paratuberkulóza diagnóza MeSH
- senzitivita a specificita MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- srovnávací studie MeSH
- Geografické názvy
- Nizozemsko MeSH
Mycobacterium avium subsp. paratuberculosis (MAP) is a vigorous microorganism which causes incurable chronic enteritis, Johne's disease (JD) in cattle. A target of control programmes for JD is to accurately detect MAP-infected cattle early to reduce disease transmission. The present study evaluated the efficacy of two different cultural procedures and a TaqMan real-time PCR assay for detection of subclinical paratuberculosis in dairy herds. Therefore, sixty-one faecal samples were collected from two Dutch dairy herds (n = 40 and n = 21, respectively) which were known to be MAP-ELISA positive. All individual samples were assessed using two different cultural protocols in two different laboratories. The first cultural protocol (first laboratory) included a decontamination step with 0.75% hexadecylpyridinium chloride (HPC) followed by inoculation on Herrold's egg yolk media (HEYM). The second protocol (second laboratory) comprised of a decontamination step using 4% NaOH and malachite green-oxalic acid followed by inoculation on two media, HEYM and in parallel on modified Löwenstein-Jensen media (mLJ). For the TaqMan real-time PCR assay, all faecal samples were tested in two different laboratories using TaqMan® MAP (Johne's) reagents (Life Technologies). The cultural procedures revealed positive reactions in 1.64% of the samples for cultivation protocol 1 and 6.56 and 8.20% of the samples for cultivation protocol 2, respectively. The results of the TaqMan real-time PCR performed in two different laboratories yielded 13.11 and 19.76% positive reaction. The kappa test showed proportional agreement 0.54 between the mLJ media (second laboratory) and TaqMan® real-time PCR method (second laboratory). In conclusion, the TaqMan real-time PCR could be a strongly useful and efficient assay for the detection of subclinical paratuberculosis in dairy cattle leading to an improvement in the efficiency of MAP control strategies.
Department of Veterinary Public Health College of Veterinary Medicine University of Mosul Mosul Iraq
GD Animal Health Service Amsbergstraat 7 Postbus 9 7400 AA Deventer The Netherlands
Zobrazit více v PubMed
J Clin Microbiol. 2003 Jul;41(7):2915-23 PubMed
Vet Rec. 2013 Oct 12;173(14 ):343 PubMed
J Clin Microbiol. 2011 Mar;49(3):893-901 PubMed
Dtsch Tierarztl Wochenschr. 2005 Aug;112(8):304-6 PubMed
J Vet Diagn Invest. 2004 Nov;16(6):503-8 PubMed
Clin Microbiol Rev. 2006 Jan;19(1):165-256 PubMed
J Vet Diagn Invest. 1999 Jul;11(4):345-51 PubMed
Crit Rev Microbiol. 2011 May;37(2):141-56 PubMed
Virulence. 2010 May-Jun;1(3):134-44 PubMed
J Vet Diagn Invest. 1995 Oct;7(4):488-93 PubMed
J Clin Microbiol. 1992 May;30(5):1134-9 PubMed
J Vet Intern Med. 2012 Nov-Dec;26(6):1239-50 PubMed
J Vet Diagn Invest. 1991 Oct;3(4):368-73 PubMed
J Clin Microbiol. 2004 Mar;42(3):1075-81 PubMed
J Clin Microbiol. 2001 Apr;39(4):1517-21 PubMed
Appl Environ Microbiol. 2005 Nov;71(11):6963-7 PubMed
Clin Diagn Lab Immunol. 2005 Jun;12(6):685-92 PubMed
Appl Environ Microbiol. 2008 May;74(9):2751-8 PubMed
Int J Food Microbiol. 2008 Dec 10;128(2):250-7 PubMed
Appl Environ Microbiol. 2012 Sep;78(18):6608-14 PubMed
J Clin Microbiol. 2013 Dec;51(12):3993-4000 PubMed
Vet Microbiol. 2011 Apr 21;149(1-2):133-8 PubMed
J Am Vet Med Assoc. 2006 Dec 15;229(12):1912-9 PubMed
Acta Vet Scand. 2014 Oct 10;56:68 PubMed
Prev Vet Med. 2007 Dec 14;82(3-4):302-7 PubMed
BMC Microbiol. 2006 Oct 04;6:87 PubMed
Rev Argent Microbiol. 2012 Jul-Sep;44(3):201-15 PubMed
BMC Res Notes. 2010 Oct 06;3:251 PubMed
Vet Microbiol. 2000 Dec 20;77(3-4):357-67 PubMed
J Microbiol Methods. 2011 Mar;84(3):413-7 PubMed
Vet Microbiol. 2009 Apr 14;136(1-2):177-9 PubMed
Prev Vet Med. 2013 Jan 1;108(1):47-62 PubMed
Vet Microbiol. 2000 Dec 20;77(3-4):387-98 PubMed
J Clin Microbiol. 1999 Jun;37(6):1746-51 PubMed
J Clin Microbiol. 2000 Dec;38(12):4463-70 PubMed
BMC Vet Res. 2012 May 02;8:49 PubMed
Prev Vet Med. 2009 Jan 1;88(1):1-14 PubMed
Vet Microbiol. 2000 Dec 20;77(3-4):253-61 PubMed
Foodborne Pathog Dis. 2004 Spring;1(1):17-26 PubMed
J Microbiol Methods. 2014 Apr;99:58-65 PubMed
Effective Control of Johne's Disease in Large Czech Dairy Herds
