Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
- Keywords
- Preterm delivery, amniotic fluid, marker, non-invasive sample,
- MeSH
- Biomarkers analysis MeSH
- Chorioamnionitis diagnosis microbiology MeSH
- Adult MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Gestational Age MeSH
- Interleukin-6 analysis MeSH
- Calreticulin analysis MeSH
- Cathepsin G analysis MeSH
- Humans MeSH
- Young Adult MeSH
- Amniotic Fluid chemistry metabolism microbiology MeSH
- Fetal Membranes, Premature Rupture metabolism microbiology MeSH
- Prospective Studies MeSH
- Sensitivity and Specificity MeSH
- Pregnancy MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Young Adult MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Biomarkers MeSH
- CALR protein, human MeSH Browser
- CTSG protein, human MeSH Browser
- Interleukin-6 MeSH
- Calreticulin MeSH
- Cathepsin G MeSH
OBJECTIVE: The study aimed to determine the cervical calreticulin and cathepsin-G concentrations in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) with respect to the presence of microbial invasion of the amniotic cavity (MIAC) and intra-amniotic inflammation (IAI). METHODS: Eighty women with singleton pregnancies complicated by PPROM were included in this study. Cervical and amniotic fluids were obtained at the time of admission, and concentrations of calreticulin and cathepsin-G in cervical fluid were determined using ELISA. The MIAC was defined as a positive PCR analysis for Ureaplasma species, Mycoplasma hominis, and/or Chlamydia trachomatis and/or by positivity for the 16S rRNA gene. IAI was defined as amniotic fluid bedside IL-6 concentrations ≥745 pg/mL Result: Neither women with MIAC nor with IAI had different cervical fluid concentrations of calreticulin (with MIAC: median 18.9 pg/mL vs. without MIAC: median 14.7 pg/mL, p = 0.28; with IAI: median 14.3 pg/mL vs. without IAI: median 15.6 pg/mL, p = 0.57;) or of cathepsin-G (with MIAC: median 30.7 pg/mL vs. without MIAC: median 24.7 pg/mL, p = 0.28; with IAI: median 27.3 pg/mL vs. without IAI: median 25.1 pg/mL, p = 0.80) than women without those complications. No associations between amniotic fluid IL-6 concentrations, gestational age at sampling, and cervical fluid calreticulin and cathepsin-G concentrations were found. CONCLUSIONS: Cervical fluid calreticulin and cathepsin-G concentrations did not reflect the presence of MIAC or IAI in women with PPROM.
Department of Obstetrics and Gynecology Sahlgrenska Academy Gothenburg University Gothenburg Sweden
f Department of Medical Genetics Peking University Health Science Center Peking China
g Biomedical Research Center University Hospital Hradec Kralove Hradec Kralove Czech Republic
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