Virulence genes and antimicrobial susceptibility of lactose-negative and lactose-positive strains of Escherichia coli isolated from pregnant women and neonates

. 2017 Sep ; 62 (5) : 363-371. [epub] 20170224

Jazyk angličtina Země Spojené státy americké Médium print-electronic

Typ dokumentu srovnávací studie, časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/pmid28236177
Odkazy

PubMed 28236177
PubMed Central PMC5579066
DOI 10.1007/s12223-017-0506-y
PII: 10.1007/s12223-017-0506-y
Knihovny.cz E-zdroje

Escherichia coli can cause serious infections in the neonates and pregnant women. Although E. coli is widely studied, E. coli lactose-negative (lac-) strains have been rarely described before. So, the aim of this study was to compare lac- and lactose-positive (lac+) E. coli strains in respect of antimicrobial susceptibility and the frequency of virulence genes (VGs). The study included 58 lac+ and 58 lac- E. coli strains isolated from pregnant women and neonates. Culture and the results of biochemical reactions were conducted for lac- and lac+ E. coli identification and differentiation. Disc diffusion test was performed to study the antimicrobial susceptibility of the isolates, and PCR was used to detect VGs. Resistance to at least one of the tested antibiotics was found among 14 (25.9%) E. coli lac+ and in 26 (44.9%) E. coli lac- strains. Both lac+ and lac- E. coli strains were mostly resistant to ampicillin (22.4 and 39.7%) and ticarcillin (20.7 and 39.7%). None of the tested strains produced extended-spectrum β-lactamases (ESBLs). Genes fimH, fimA, iutA, sfa/foc, neuC, ibeA, and hlyF were detected, respectively, in 96.6, 82.8, 32.8, 24.1, 22.4, 12.1, and 6.9% of lac+ E. coli strains and in 94.8, 86.2, 48.3, 19.0, 8.6, 8.6, and 1.7% of lac- strains. The antimicrobial susceptibility and the pathogenic potential of both tested groups of E. coli strains are similar. Therefore, omitting E. coli lac- strains as a potential etiological agent of infections may pose a threat to the health and life of both mothers and neonates.

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Al-Mayahie SM. 2013 Vaginal colonization by pap G allele II+ Escherichia coli isolates from pregnant and nonpregnant women as predisposing factor to pyelonephritis. Infect Dis Obstet Gynecol ; doi:10.1155/2013/860402 PubMed PMC

Alemu A, Moges F, Shiferaw Y, Tafess K, Kassu A, Anagaw B, Agegn A. Bacterial profile and drug susceptibility pattern of urinary tract infection in pregnant women at University of Gondar Teaching Hospital, Northwest Ethiopia. BMC Res Notes. 2012;5:197. doi: 10.1186/1756-0500-5-197. PubMed DOI PMC

Barber AE, Norton JP, Spivak AM, Mulvey MA. Urinary tract infections: current and emerging management strategies. Clin Infect Dis. 2013;57:719–724. doi: 10.1093/cid/cit284. PubMed DOI PMC

Barcaite E, Bartusevicius A, Tameliene R, Maleckiene L, Vitkauskiene A, Nadisauskiene R. Group B Streptococcus and Escherichia coli colonization in pregnant women and neonates in Lithuania. Int J Gynaecol Obstet. 2012;117:69–73. doi: 10.1016/j.ijgo.2011.11.016. PubMed DOI

Cisowska A, Ruczkowska J, Doroszkiewicz W. Wrażliwość na antybiotyki szczepów Escherichia coli z otoczkowym antygenem K1 izolowanych z zakażeń układu moczowego. Med Dośw Mikrobiol. 2001;53:345–355.

Daniluk T, Ściepuk M, Fiedoruk K, Zaremba ML, Rożkiewicz D, Ołdak E. Częstość występowania i wrażliwość na antybiotyki szczepów Escherichia coli i Klebsiella pneumoniae izolowanych od dzieci hospitalizowanych w Uniwersyteckim Szpitalu Dziecięcym w latach 2004–2007. Now Lek 2008; 77:265–72 (article in Polish). http://www.nowinylekarskie.ump.edu.pl/uploads/2008/4/265_4_77_2008.pdf Accessed 30 September 2015

Enayat K, Fariba F, Bahram N (2008) Asymptomatic bacteriuria among pregnant women referred to outpatient clinics in Sanandaj, Iran. Int Braz J Urol 34 (6):699–707 PubMed

Ewers C, Li G, Wilking H, Kieβling S, Alt K, Antáo EM, Laturnus C, Diehl I, Glodde S, Homeier T, Böhnke U, Steinrück H, Philipp HC, Wieler LH. Avian pathogenic, uropathogenic, and newborn meningitis-causing Escherichia coli: how closely related are they? Int J Med Microbiol. 2007;297:163–176. doi: 10.1016/j.ijmm.2007.01.003. PubMed DOI

Gadage D, Wankhade A, Muley VV, Paralikar AV, Bhore AV. Are inactive E. coli always commensals? Sch J App Med Sci. 2014;2:426–427.

Hilbert DW, Paulish TE, Mordechai E, Adelson ME, Gygax SE, Trama JP. Antimicrobial non-susceptibility of cervico-vaginal and rectal Escherichia coli isolates is associated with phylogeny and plasmid carriage. Eur J Clin Microbiol Infect Dis. 2009;28:1399–1403. doi: 10.1007/s10096-009-0788-3. PubMed DOI

Janicka G, Wojciechowska D, Hareńska K, Porada J, Kłyszejko C. The resistance to betalactam antibiotics of lactose-positive and lactose-negative strains of Escherichia coli. Acta Microbiol Pol. 1997;46:399–403. PubMed

Jarlier V, Nicolas M, Fourier G, Philippon A. Extended broad-spectrum beta-lactamases conferring transferable resistance to newer beta-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev Infect Dis. 1988;10:867–878. doi: 10.1093/clinids/10.4.867. PubMed DOI

Johnson J. Virulence factors in Escherichia coli urinary tract infection. Clin Microbiol Rev. 1991;4:80–128. doi: 10.1128/CMR.4.1.80. PubMed DOI PMC

Juskova E, Ciznar I. Occurrence of genes for P and S fimbriae and hemolysin in urinary Escherichia coli. Folia Microbiol. 1994;39:159–161. doi: 10.1007/BF02906814. PubMed DOI

Kaczmarek A, Budzyńska A, Gospodarek E. Antimicrobial sensitivity of Escherichia coli straind with K1 antigen isolated from pregnant women and newborns. Med Dosw Mikrobiol. 2011;63:121–130. PubMed

Kaczmarek A, Budzyńska A, Gospodarek E. Prevalence of genes encoding virulence factors among Escherichia coli with K1 antigen and non-K1 E. coli strains. J Med Microbiol. 2012;61:1360–1365. doi: 10.1099/jmm.0.044263-0. PubMed DOI

Korczak B, Frey J, Schrenzel J, Schrenzel J, Pluschke G, Pfister R, Ehricht R, Kuhnert P. Use of diagnostic microarrays for determination of virulence gene patterns of Escherichia coli K1, a major cause of neonatal meningitis. J Clin Microbiol. 2005;43:1024–1031. doi: 10.1128/JCM.43.3.1024-1031.2005. PubMed DOI PMC

Lina TT, Khajanchi BK, Azmi IJ, Islam MA, Mahmood B, Akter M, Banik A, Alim R, Navarro A, Perez G, Cravioto A, Talukder KA, et al. Phenotypic and molecular characterization of extended-spectrum beta-lactamase-producing Escherichia coli in Bangladesh. PLoS One. 2014;9:e108735. doi: 10.1371/journal.pone.0108735. PubMed DOI PMC

Mehta M, Bhardwaj S, Sharma J. Prevalence and antibiotic susceptibility pattern of multi-drug resistant Escherichia coli isolates from urinary tract infection (UTI) patients. Int J Life Sci Pharm Res. 2012;2:6–11.

Micenková L, Štaudová B, Bosák J, Mikalová L, Littnerová S, Vrba M, Ševčíková A, Woznicová V, Šmajs D. Bacteriocin-encoding genes and ExPEC virulence determinants are associated in human fecal Escherichia coli strains. BMC Microbiol. 2014;14:109. doi: 10.1186/1471-2180-14-109. PubMed DOI PMC

Newton ER. Preterm labor, preterm premature rupture of membranes, and chorioamnionitis. Clin Perinatol. 2005;32:571–600. doi: 10.1016/j.clp.2005.05.001. PubMed DOI

Nicoletti M, Superti F, Conti C, Calconi A, Zagaglia C. Virulence factors of lactose-negative Escherichia coli strains isolated from children with diarrhea in Somalia. J Clin Microbiol. 1988;26:524–529. PubMed PMC

Obata-Yasuoka M, Ba-Thein W, Tsukamoto T, Yoshikawa H, Hayashi H. Vaginal Escherichia coli share common virulence factor profiles, serotypes and phylogeny with other extraintestinal E. coli. Microbiology. 2002;148:2745–2752. doi: 10.1099/00221287-148-9-2745. PubMed DOI

Poey ME, Albini M, Saona G, Laviña M. Virulence profiles in uropathogenic Escherichia coli isolated from pregnant women and children with urinary tract abnormalities. Microb Pathog. 2012;52:292–301. doi: 10.1016/j.micpath.2012.02.006. PubMed DOI

Sękowska A, Ibsz-Fijałkowska A, Gołdyn K, Gospodarek E. Wrażliwość na wybrane tetracykliny pałeczek z rodziny Enterobacteriaceae. Med Dośw Mikrobiol. 2009;61:321–326. PubMed

Sobieszczańska B, Kowalska-Krochmal B, Mowszet K, Pytrus T. Wrażliwość na antybiotyki i chemioterapeutyki enteroagregacyjnych szczepów Escherichia coli (EAEC) izolowanych od dzieci z biegunką. Przegl Epidemiol. 2003;57:499–503. PubMed

Spaetgens R, DeBella K, Ma D, Robertson S, Mucenski M, Davies HD. Perinatal antibiotic usage and changes in colonization and resistance rates of group B Streptococcus and other pathogens. Obstet Gynecol. 2002;100:525–533. PubMed

Tadesse E, Teshome M, Merid Y, Kibret B, Shimelis T. Asymptomatic urinary tract infection among pregnant women attending the antenatal clinic of Hawassa Referral Hospital. Southern Ethiopia BMC Res Notes. 2014;17:155. doi: 10.1186/1756-0500-7-155. PubMed DOI PMC

Tamelienė R, Barčaitė E, Stonienė D, Buinauskienė J, Markūnienė E, Kudrevičienė A, Vitkauskienė A, Jomantienė D, Nadišauskienė R. Escherichia coli colonization in neonates: prevalence, perinatal transmission, antimicrobial susceptibility, and risk factors. Medicina (Kaunas) 2012;48:71–76. PubMed

Tenaillon O, Skurnik D, Picard B, Denamur E. The population genetics of commensal Escherichia coli. Nat Rev Microbiol. 2010;8:207–217. doi: 10.1038/nrmicro2298. PubMed DOI

The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 3.1, 2013. http://www.eucast.org. Accessed 30September 2015

Villar HE, Aubert V, Baserni MN, Jugo MB. Maternal carriage of extended-spectrum beta-lactamase-producing Escherichia coli isolates in Argentina. J Chemother. 2013;25:324–327. doi: 10.1179/1973947813Y.0000000081. PubMed DOI

Watt S, Lanotte P, Mereghetti L, Moulin-Schouleur M, Picard B, Quentin R. Escherichia coli strains from pregnant women and neonates: intraspecies genetic distribution and prevalence of virulence factors. J Clin Microbiol. 2003;41:1929–1935. doi: 10.1128/JCM.41.5.1929-1935.2003. PubMed DOI PMC

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