Beneficial effect of adipokinetic hormone on neuromuscular paralysis in insect body elicited by braconid wasp venom
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
28257925
DOI
10.1016/j.cbpc.2017.02.011
PII: S1532-0456(17)30051-0
Knihovny.cz E-resources
- Keywords
- AKH, Akh gene expression, Habrobracon hebetor, Metabolism, Paralysis, Q-PCR, Stress, Venom,
- MeSH
- Allostasis MeSH
- Biomarkers metabolism MeSH
- Central Nervous System drug effects metabolism MeSH
- Energy Metabolism drug effects MeSH
- Hemolymph drug effects metabolism MeSH
- Heteroptera drug effects physiology MeSH
- Insect Hormones agonists genetics metabolism physiology MeSH
- Thorax MeSH
- Injections MeSH
- Kinetics MeSH
- Pyrrolidonecarboxylic Acid agonists analogs & derivatives MeSH
- Neuromuscular Junction drug effects physiology MeSH
- Neuropeptides agonists analysis genetics metabolism physiology MeSH
- Oligopeptides agonists genetics metabolism physiology MeSH
- Paralysis chemically induced veterinary MeSH
- Up-Regulation drug effects MeSH
- Wasp Venoms antagonists & inhibitors isolation & purification toxicity MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- adipokinetic hormone MeSH Browser
- Biomarkers MeSH
- Insect Hormones MeSH
- Pyrrolidonecarboxylic Acid MeSH
- Neuropeptides MeSH
- Oligopeptides MeSH
- Pea-CAH-II neuropeptide MeSH Browser
- Wasp Venoms MeSH
The effect of Habrobracon hebetor venom and the role of the adipokinetic hormone (AKH) in poisoned adult females of the firebug Pyrrhocoris apterus were studied 24 and 48h after treatments. Venom application elicited total neuromuscular paralysis in firebugs, but the co-application of venom and Pyrap-AKH significantly reduced paralysis (up to 3.2 times) compared to the application of venom only. Although the mechanisms of their action are unknown, both agents might affect neuromuscular junctions. Venom application significantly increased the expression of both P. apterus Akh genes (Pyrap-Akh 5.4 times and Peram-Cah-II 3.6 times), as well as the level of AKHs in the central nervous system (2.5 times) and haemolymph (3.0 times). In the haemolymph, increased AKH levels might have led to the mobilization of stored lipids, which increased 1.9 times, while the level of free carbohydrates remained unchanged. Total metabolism, monitored by carbon dioxide production, significantly declined in paralysed P. apterus individuals (1.4 times and 1.9 times, 24 and 48h after the treatment, respectively), probably because of a malfunction of the muscular system. The results suggest an active role of AKH in the defence mechanism against the stress elicited by neuromuscular paralysis, and the possible involvement of this hormone in neuronal/neuromuscular signalling.
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