Prostaglandin E2 stimulates the expression of cumulus expansion-related genes in pigs: the role of protein kinase B
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
28389370
DOI
10.1016/j.prostaglandins.2017.04.001
PII: S1098-8823(16)30156-3
Knihovny.cz E-zdroje
- Klíčová slova
- Cumulus, Oocyte, Prostaglandin E2, Protein kinase B,
- MeSH
- aktivace enzymů účinky léků MeSH
- dinoproston farmakologie MeSH
- down regulace účinky léků MeSH
- kumulární buňky cytologie účinky léků metabolismus MeSH
- mitogenem aktivovaná proteinkinasa 1 metabolismus MeSH
- mitogenem aktivovaná proteinkinasa 3 metabolismus MeSH
- oocyty cytologie účinky léků MeSH
- prasata MeSH
- protoonkogenní proteiny c-akt metabolismus MeSH
- regulace genové exprese účinky léků MeSH
- upregulace účinky léků MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- dinoproston MeSH
- mitogenem aktivovaná proteinkinasa 1 MeSH
- mitogenem aktivovaná proteinkinasa 3 MeSH
- protoonkogenní proteiny c-akt MeSH
The production of prostaglandin E2 (PGE2) seems to play an important role in the ovulation process. PGE2 was found to induce cumulus expansion and meiosis resumption in mice, but little is known about its role in pigs. The goals of this study were (a) to assess the effect of PGE2 on the expression levels of cumulus expansion-related genes, (b) to define the signaling pathways that drive the PGE2-stimulated expression of cumulus expansion-related genes, (c) to measure the effect of PGE2 on the activation of key signaling molecules (MAPK3/1, PKB) and on hyaluronan production in cumulus cells, and (d) to assess the effect of PGE2 on meiosis resumption. We documented that PGE2 is able to induce the expression of cumulus expansion-related genes (HAS2, TNFAIP6) as well as genes involved in steroidogenesis (CYP11A1) or prostaglandin production (PTGS2). PGE2 is able to activate PKB and MAPK3/1 and induce mild cumulus expansion and meiosis resumption, but less efficiently than FSH.
Citace poskytuje Crossref.org
A Role of PI3K/Akt Signaling in Oocyte Maturation and Early Embryo Development
