Prostaglandin E2 stimulates the expression of cumulus expansion-related genes in pigs: the role of protein kinase B
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
28389370
DOI
10.1016/j.prostaglandins.2017.04.001
PII: S1098-8823(16)30156-3
Knihovny.cz E-resources
- Keywords
- Cumulus, Oocyte, Prostaglandin E2, Protein kinase B,
- MeSH
- Enzyme Activation drug effects MeSH
- Dinoprostone pharmacology MeSH
- Down-Regulation drug effects MeSH
- Cumulus Cells cytology drug effects metabolism MeSH
- Mitogen-Activated Protein Kinase 1 metabolism MeSH
- Mitogen-Activated Protein Kinase 3 metabolism MeSH
- Oocytes cytology drug effects MeSH
- Swine MeSH
- Proto-Oncogene Proteins c-akt metabolism MeSH
- Gene Expression Regulation drug effects MeSH
- Up-Regulation drug effects MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Dinoprostone MeSH
- Mitogen-Activated Protein Kinase 1 MeSH
- Mitogen-Activated Protein Kinase 3 MeSH
- Proto-Oncogene Proteins c-akt MeSH
The production of prostaglandin E2 (PGE2) seems to play an important role in the ovulation process. PGE2 was found to induce cumulus expansion and meiosis resumption in mice, but little is known about its role in pigs. The goals of this study were (a) to assess the effect of PGE2 on the expression levels of cumulus expansion-related genes, (b) to define the signaling pathways that drive the PGE2-stimulated expression of cumulus expansion-related genes, (c) to measure the effect of PGE2 on the activation of key signaling molecules (MAPK3/1, PKB) and on hyaluronan production in cumulus cells, and (d) to assess the effect of PGE2 on meiosis resumption. We documented that PGE2 is able to induce the expression of cumulus expansion-related genes (HAS2, TNFAIP6) as well as genes involved in steroidogenesis (CYP11A1) or prostaglandin production (PTGS2). PGE2 is able to activate PKB and MAPK3/1 and induce mild cumulus expansion and meiosis resumption, but less efficiently than FSH.
References provided by Crossref.org
A Role of PI3K/Akt Signaling in Oocyte Maturation and Early Embryo Development
