Potential Involvement of SCF-Complex in Zygotic Genome Activation During Early Bovine Embryo Development
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Immunofluorescence, In situ proximity ligation assay, Preimplantation development, SCF-complex, Western blot, qRT-PCR,
- MeSH
- Transcriptional Activation MeSH
- Embryonic Development * MeSH
- SKP Cullin F-Box Protein Ligases metabolism MeSH
- Proteolysis MeSH
- Cattle MeSH
- Gene Expression Profiling MeSH
- Ubiquitin-Protein Ligases metabolism MeSH
- Gene Expression Regulation, Developmental MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- SKP Cullin F-Box Protein Ligases MeSH
- Ubiquitin-Protein Ligases MeSH
Proper timing of degradation of maternal protein reserves is important for early embryonic development. The major modification that triggers proteins to degradation is ubiquitination, mediated by ubiquitin-proteolytic system. We focus here on Skp 1-Cul 1-F-box complex (SCF-complex), E3 ubiquitin-ligase, a part of ubiquitin-proteolytic system, which transfer ubiquitin to the substrate protein. We describe in this chapter the methods for the characterization of the expression profile of mRNA and protein of invariant members of SCF-complex and for the definition of SCF-complex activity.
Department of Veterinary Sciences Czech University of Life Sciences Prague Prague Czech Republic
Faculty of Science Charles University Prague Prague Czech Republic
References provided by Crossref.org
The neglected part of early embryonic development: maternal protein degradation
