Potential Involvement of SCF-Complex in Zygotic Genome Activation During Early Bovine Embryo Development
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Immunofluorescence, In situ proximity ligation assay, Preimplantation development, SCF-complex, Western blot, qRT-PCR,
- MeSH
- aktivace transkripce MeSH
- embryonální vývoj * MeSH
- proteinligasy komplexu SCF metabolismus MeSH
- proteolýza MeSH
- skot MeSH
- stanovení celkové genové exprese MeSH
- ubikvitinligasy metabolismus MeSH
- vývojová regulace genové exprese MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- proteinligasy komplexu SCF MeSH
- ubikvitinligasy MeSH
Proper timing of degradation of maternal protein reserves is important for early embryonic development. The major modification that triggers proteins to degradation is ubiquitination, mediated by ubiquitin-proteolytic system. We focus here on Skp 1-Cul 1-F-box complex (SCF-complex), E3 ubiquitin-ligase, a part of ubiquitin-proteolytic system, which transfer ubiquitin to the substrate protein. We describe in this chapter the methods for the characterization of the expression profile of mRNA and protein of invariant members of SCF-complex and for the definition of SCF-complex activity.
Department of Veterinary Sciences Czech University of Life Sciences Prague Prague Czech Republic
Faculty of Science Charles University Prague Prague Czech Republic
Citace poskytuje Crossref.org
The neglected part of early embryonic development: maternal protein degradation
