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Identification of calmodulin binding proteins in the entomopathogenic fungus Beauveria bassiana

Kim, Jiyoung
Author Kim, Jiyoung Institute for Bio-Medical Convergence, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University, Incheon, 404-834, South Korea. jiyoungk1972@gmail.com
Oh, Junsang
Author Oh, Junsang College of Pharmacy, Chung-Ang University, Seoul, South Korea
Yoon, Deok-Hyo
Author Yoon, Deok-Hyo Institute for Bio-Medical Convergence, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University, Incheon, 404-834, South Korea
Sung, Gi-Ho
Author Sung, Gi-Ho Institute for Bio-Medical Convergence, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University, Incheon, 404-834, South Korea. sung97330@gmail.com

Folia microbiologica. 2018 Jan ; 63 (1) : 13-16. [epub] 20170511

Folia Microbiol (Praha)
ISSN 1874-9356 | 0015-5632
Source

Language English Country United States Media print-electronic

Document type Journal Article

Persistent link https://www.medvik.cz/link/pmid28497337

Grant support
PJ011949 Rural Development Administration, Korea

PubMed 28497337
DOI 10.1007/s12223-017-0529-4
PII: 10.1007/s12223-017-0529-4
Knihovny.cz E-resources

MeSH
Adenosine Triphosphate metabolism MeSH
Beauveria chemistry genetics metabolism MeSH
Fungal Proteins chemistry genetics metabolism MeSH
Insecta microbiology MeSH
Calmodulin chemistry genetics metabolism MeSH
Calmodulin-Binding Proteins chemistry genetics metabolism MeSH
Proteomics MeSH
Amino Acid Sequence MeSH
Sequence Alignment MeSH
Tandem Mass Spectrometry MeSH
Protein Binding MeSH
Animals MeSH
Check Tag
Animals MeSH
Publication type
Journal Article MeSH
Names of Substances
Adenosine Triphosphate MeSH
Fungal Proteins MeSH
Calmodulin MeSH
Calmodulin-Binding Proteins MeSH

Calmodulin (CaM) is a primary Ca2+ receptor and plays a pivotal role in a variety of cellular responses in eukaryotes. Even though a large number of CaM-binding proteins are well known in yeast, plants, and animals, little is known regarding CaM-targeted proteins in filamentous fungi. To identify CaM-binding proteins in filamentous fungi, we used a proteomics method coupled with co-immunoprecipitation (CoIP) and MALDI-TOF/TOF mass spectrometry (MS) in Beauveria bassiana. Through this method, we identified ten CaM-binding proteins in B. bassiana. One of the CaM-targeted proteins was the heat shock protein 70 (BbHSP70) in B. bassiana. Our biochemical study showed that ATP inhibits the molecular interaction between BbHSP70 and CaM, suggesting a regulatory mechanism between CaM and ATP for regulating BbHSP70.

College of Pharmacy Chung Ang University Seoul South Korea

Institute for Bio Medical Convergence International St Mary's Hospital and College of Medicine Catholic Kwandong University Incheon 404 834 South Korea

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