BACKGROUND: Azorella compacta is traditionally used in the form of tea (infusion), in the Andean region of South America, to treat various chronic diseases. However, the health-promoting properties of this herbal tea have not yet been extensively explored. MATERIALS AND METHODS: The free radical scavenging activity of A. compacta infusion (ACI) was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and superoxide anion radical assays. The activation of immune cells by ACI, as determined by cell surface cluster of differentiation 69 expression, was measured by flow cytometry. The qualitative polyphenolic composition of ACI was investigated by HPLC/PDA/ESI-MS, (High-performance liquid chromatography coupled with photodiode array detection and electrospray ionization - mass spectrometry) and the total content of polyphenols was estimated by spectrophotometric methods. RESULTS: Eight polyphenols including chlorogenic acid, 6,8-di-C-hexosyl apigenin, isoorientin, orientin, dicaffeoylquinic acid, biochanin A-O-glucoside, biochanin A-O-(malonyl)-glucoside, and licoisoflavone A were tentatively identified in ACI. The total contents of phenols, flavonoids, and tannins in lyophilized ACI were 5.40 mg/100 mg ACI, 1.79 mg/100 mg ACI, and 1.76 mg/100 mg ACI, respectively. ACI, within the range of 25-400 μg/mL, scavenged DPPH and O2.- by 15-90% and 20-88%, respectively. The human natural killer (NK) cells were substantially activated by ACI, whereas T cells and granulocytes were slightly stimulated. CONCLUSION: Overall, the results demonstrate the free radical scavenging and immune-stimulating properties of ACI, and support, at least in part, its potential utilization as a functional herbal tea. for preventing chronic diseases and as a nonspecific immune stimulator during human immunosenescence. SUMMARY: The total contents of phenols, flavonoids, and tannins in Azorella compacta infusion (ACI) were 5.40 mg/100 mg ACI, 1.79 mg/100 mg ACI, and 1.76 mg/100 mg ACI, respectively.Eight polyphenols including chlorogenic acid, 6,8-di-C-hexosyl apigenin, isoorientin, orientin, dicaffeoylquinic acid, biochanin A-O-glucoside, biochanin A-O-(malonyl)-glucoside, and licoisoflavone A were tentatively identified in ACI by HPLC/PDA/ESI-MS.ACI, within the range of 25-400 μg/ml, scavenged 1,1-diphenyl-2-picrylhydrazyl (DPPH) and O2. by 15-90% and 20-88%, respectively.The human natural killer (NK) cells were substantially activated by ACI, whereas T cells and granulocytes were slightly stimulated. Abbreviations used: ESI: electrospray ionization, HPLC: high performance liquid chromatography, PDA: photodiode array detector, MS: mass spectrometry, MS/MS: tandem mass spectrometry, MW: molecular weight, m/z: mass-to-charge ratio, FITC: fluorescent isothiocyanate, PE: phycoerythrin.

Department of Pharmacognosy Faculty of Pharmacy Charles University Heyrovského Hradec Králové Czech Republic

Department of Pharmacology and Toxicology Faculty of Pharmacy Charles University Heyrovského Hradec Králové Czech Republic

Departmento de Química Universidad Andrés Bello Viña del Mar Chile

Institute of Clinical Immunology and Allergology Faculty of Medicine and University Hospital Charles University Sokolská Hradec Králové Czech Republic

Laboratory of Algal Biotechnology Centre ALGATECH Institute of Microbiology of the Czech Academy of Sciences Opatovický mlýn Třeboň Czech Republic

Zobrazit více v PubMed

Wickens GE. Llareta (Azorella compacta, Umbelliferae): A review. Econ Bot. 1995;49:207–12.

Molina-Salinas GM, Bórquez J, Said-Fernández S, Loyola LA, Yam-Puc A, Becerril-Montes P, et al. Antituberculosis activity of alkylated mulinane diterpenoids. Fitoterapia. 2010;81:219–22. PubMed

Scheibmeir HD, Christensen K, Whitaker SH, Jegaethesan J, Clancy R, Pierce JD. A review of free radicals and antioxidants for critical care nurses. Intens Crit Care Nurs. 2005;21:24–8. PubMed

Cheel J, Antwerpen PV, Tůmová L, Onofre G, Vokurková D, Zouaoui-Boudjeltia K, et al. Free radical-scavenging, antioxidant and immunostimulating effects of a licorice infusion (Glycyrrhiza glabra L.) Food Chem. 2010;122:508–17.

Brush J, Mendenhall E, Guggenheim A, Chan T, Connelly E, Soumyanath A, et al. The effect of Echinacea purpurea, Astragalus membranaceus and Glycyrrhiza glabra on CD69 expression and immune cell activation in humans. Phytother Res. 2006;20:687–95. PubMed

Rojo LE, Benites J, López J, Rojas M, Díaz P, Ordónez J, et al. Antioxidant capacity and polyphenolic content of twelve traditionally used herbal medicinal infusions from the South American Andes. Bol Latinoam Caribe Plant Med Aromát. 2009;8:498–508.

Quesada L, Gutierrez M, Astudillo L, San-Martín A, Fuentes E, Palomo I, et al. Determination of antibacterial, antioxidant, antiplatelet and inhibition of cholinesterase activities from the methanolic extracts of Azorella species (Apiaceae) Bol Latinoam Caribe Plant Med Aromát. 2013;12:99–107.

Bórquez J, Kennelly EJ, Simirgiotis MJ. Activity guided isolation of isoflavones and hyphenated HPLC-PDA-ESI-ToF-MS metabolome profiling of Azorella madreporica Clos from northern Chile. Food Res Int. 2013;52:288–97.

Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39:44–84. PubMed

Ziegler SF, Ramsdell F, Alderson MR. The activation antigen CD69. Stem Cells. 1994;12:456–65. PubMed

Cheel J, Onofre G, Vokurkova D, TŮmová L, Neugebauerová J. Licorice infusion: Chemical profile and effects on the activation and the cell cycle progression of human lymphocytes. Pharmacogn Mag. 2010;6:26–33. PubMed PMC

Wagner H, Jurcic K. Immunological studies of Revitonil®, a phytopharmaceutical containing Echinacea purpurea and Glycyrrhiza glabra root extract. Phytomedicine. 2002;9:390–7. PubMed

Zwickey H, Brush J, Iacullo CM, Connelly E, Gregory WL, Soumyanath A, et al. The effect of Echinacea purpurea, Astragalus membranaceus and Glycyrrhiza glabra on CD25 expression in humans: A pilot study. Phytother Res. 2007;21:1109–12. PubMed

Jaiswal R, Müller H, Müller A, Karar MGE, Kuhnert N. Identification and characterization of chlorogenic acids, chlorogenic acid glycosides and flavonoids from Lonicera henryi L (Caprifoliaceae) leaves by LC-MSn. Phytochemistry. 2014;108:252–63. PubMed

Clifford MN, Knight S, Kuhnert N. Discriminating between the six isomers of Dicaffeoylquinic acid by LC-MSn. J Agric Food Chem. 2005;53:3821–32. PubMed

Carini M, Aldini G, Furlanetto S, Stefani R, Facino RM. LC coupled to ion-trap MS for the rapid screening and detection of polyphenol antioxidants from Helichrysum stoechas. J Pharm Biomed Anal. 2001;24:517–26. PubMed

Ferreres F, Silva BM, Andrade PB, Seabra RM, Ferreira MA. Approach to the study of C-glycosyl flavones by ion trap HPLC-PAD-ESI/MS/MS: Application to seeds of quince (Cydonia oblonga) Phytochem Anal. 2003;14:352–9. PubMed

Cuyckens F, Claeys M. Mass spectrometry in the structural analysis of flavonoids. J Mass Spectrom. 2004;39:1–15. PubMed

Sun Y, Zhang X, Xue X, Zhang Y, Xiao H, Liang X. Rapid identification of polyphenol C-glycosides from Swertia franchetiana by HPLC-ESI-MS-MS. J Chromatogr Sci. 2009;47:190–6. PubMed

Costa GM, Ortmann CF, Schenkel EP, Reginatto FH. An HPLC-DAD method to quantification of main phenolic compounds from leaves of Cecropia species. J Braz Chem Soc. 2011;22:1096–102.

Yang J, Kwon YS, Kim MJ. Isolation and characterization of bioactive compounds from Lepisorus thunbergianus (Kaulf.) Arab J Chem. 2015;8:407–13.

Andrade-Cetto A, Wiedenfeld H. Hypoglycemic effect of Cecropia obtusifolia on streptozotocin diabetic rats. J Ethnopharmacol. 2001;78:145–9. PubMed

Antioxidant, Antidiabetic, and Anticholinesterase Activities and Phytochemical Profile of Azorella glabra Wedd