Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
28651197
DOI
10.1016/j.jhazmat.2017.06.035
PII: S0304-3894(17)30456-9
Knihovny.cz E-resources
- Keywords
- Antioxidants, Fluorescence microscopy, Heavy metals, Oxidative stress, RT-PCR,
- MeSH
- Antioxidants pharmacology MeSH
- Ascorbate Peroxidases genetics MeSH
- Chlorophyll metabolism MeSH
- Chlorophyta drug effects genetics metabolism MeSH
- Ascorbic Acid pharmacology MeSH
- Lead toxicity MeSH
- Oxidative Stress drug effects MeSH
- Oxidoreductases genetics MeSH
- Mercury toxicity MeSH
- Superoxide Dismutase metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antioxidants MeSH
- Ascorbate Peroxidases MeSH
- Chlorophyll MeSH
- glutathione dehydrogenase (ascorbate) MeSH Browser
- Ascorbic Acid MeSH
- Lead MeSH
- Oxidoreductases MeSH
- Mercury MeSH
- Superoxide Dismutase MeSH
Impact of exogenous ascorbic acid (AsA, 100μM) on acute metal toxicity (Pb and Hg, 24h of exposure to 100μM) in unicellular green alga Coccomyxa subellipsoidea was studied. Hg (often extensively) depleted amount of pigments, potassium, soluble proteins, endogenous AsA, non-protein thiols and nitric oxide signal but elevated ROS signal and activities of catalase and superoxide dismutase. Responses to Pb application (if any) were less visible with ROS signal being slightly elevated and amount of non-protein thiols being slightly reduced. Exogenous AsA considerably improved mainly Hg-induced damage at the level of oxidative stress and physiological parameters and evoked an increase in nitric oxide signal. Maximum amount of total Hg was almost 4-fold higher than that of Pb (36.5 vs. 9.73mg/g DW) and AsA depleted both total and intracellular accumulation. At the level of ascorbate-related proteins, exogenous AsA suppressed metal-induced expression and activity of MDHAR and APX but not of DHAR, which may indicate a role of DHAR in algal stress tolerance. Our data suggest that given Coccomyxa species is suitable candidate for remediation of Hg or Pb and that ascorbic acid effectively ameliorates metal-induced toxicity without side effects.
References provided by Crossref.org
Metal contamination - a global environmental issue: sources, implications & advances in mitigation
Mercury Content and Amelioration of Its Toxicity by Nitric Oxide in Lichens
Impact of humic acid on the accumulation of metals by microalgae
