Origin of enhanced VCD in amyloid fibril spectra: Effect of deuteriation and pH
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem
Grantová podpora
R01 AG033719
NIA NIH HHS - United States
PubMed
28710791
DOI
10.1002/chir.22722
Knihovny.cz E-zdroje
- Klíčová slova
- VCD intensity enhancement origin, deuterium exchange, protein fibrils, supramolecular chirality, vibrational circular dichroism,
- MeSH
- amyloid chemie MeSH
- cirkulární dichroismus * MeSH
- deuterium chemie MeSH
- inzulin chemie MeSH
- koncentrace vodíkových iontů MeSH
- lidé MeSH
- muramidasa chemie MeSH
- proteinové agregáty * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Názvy látek
- amyloid MeSH
- deuterium MeSH
- inzulin MeSH
- muramidasa MeSH
- proteinové agregáty * MeSH
Supramolecular chirality of amyloid fibrils, protein aggregates related to many neurodegenerative diseases, is a remarkable property associated with fibril structure and polymorphism. Since its discovery almost 10 years ago there is still little understanding of this phenomenon, including the cause of the highly enhanced vibrational circular dichroism (VCD) intensity arising from fibril supramolecular chirality. In this study, VCD spectra, enhanced by filament supramolecular chirality, are presented for lysozyme and insulin fibrils above and below pH 2 and after deuterium exchange, above and below pD 2. Supramolecular chirality (observed by VCD) and fibril morphology (documented by atomic force microscopy) are not affected by protein deuteriation. In D2 O the fibril VCD sign pattern changes to fewer bands, with implications for the amide I/II origin of enhanced VCD intensity. Separation of amide I and II signals will facilitate calculations of enhanced VCD spectra of amyloid fibrils and enable a better understanding of the origin of the VCD sign pattern.
BioTools Inc Jupiter Florida USA
Department of Chemistry Syracuse University Syracuse New York USA
Department of Chemistry University at Albany SUNY Albany New York USA
Institute of Physics Faculty of Mathematics and Physics Charles University Prague 2 Czech Republic
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