Genes of cellular components of morphogenesis in porcine oocytes before and after IVM
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
28733345
DOI
10.1530/rep-17-0367
PII: REP-17-0367
Knihovny.cz E-zdroje
- MeSH
- buněčná adheze genetika MeSH
- buněčná diferenciace genetika MeSH
- fenotyp MeSH
- genotyp MeSH
- IVM techniky * MeSH
- kultivované buňky MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- morfogeneze genetika MeSH
- oocyty metabolismus MeSH
- pohyb buněk genetika MeSH
- proliferace buněk genetika MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
- stanovení celkové genové exprese metody MeSH
- Sus scrofa MeSH
- transkriptom MeSH
- vývojová regulace genové exprese * MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Proper oocyte maturation in mammals produces an oocyte capable of monospermic fertilization and embryo preimplantation. The cumulus-oocyte complexes (COCs), surrounding an oocyte, play a significant role in oocyte maturation. During this process, when the COCs undergo cumulus expansion wherein tightly compact cumulus cells (CCs) form a dispersed structure, permanent biochemical and molecular modifications occur in the maturing oocytes, indicating that the gene expression between immature and mature oocytes differs significantly. This study focuses on the genes responsible for the cellular components of morphogenesis within the developing oocyte. Brilliant cresyl blue (BCB) was used to determine the developmental capability of porcine oocytes. The immature oocytes (GV stage) were compared with matured oocytes (MII stage), using microarray and qRT-PCR analysis to track changes in the genetic expression profile of transcriptome genes. The data showed substantial upregulation of genes influencing oocyte's morphology, cellular migration and adhesion, intracellular communication, as well as plasticity of nervous system. Conversely, downregulation involved genes related to microtubule reorganization, regulation of adhesion, proliferation, migration and cell differentiation processes in oocytes. This suggests that most genes recruited in morphogenesis in porcine oocyte in vitro, may have cellular maturational capability, since they have a higher level of expression before the oocyte's matured form. It shows the process of oocyte maturation and developmental capacity is orchestrated by significant cellular modifications during morphogenesis.
Department of AnatomyPoznan University of Medical Sciences Poznan Poland
Department of Histology and EmbryologyPoznan University of Medical Sciences Poznan Poland
Department of Histology and EmbryologyWroclaw Medical University Wroclaw Poland
Institute of Veterinary SciencesPoznan University of Life Sciences Poznan Poland
Citace poskytuje Crossref.org
Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM
