Molecular characterization and heterogeneity of circulating tumor cells in breast cancer
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
ST.C280.17.010
Minister of Science and Higher Education.
PubMed
28815327
PubMed Central
PMC5680374
DOI
10.1007/s10549-017-4452-9
PII: 10.1007/s10549-017-4452-9
Knihovny.cz E-zdroje
- Klíčová slova
- Breast cancer, CTCs, Circulating tumor cells, Cultivation, Gene expression, In vitro, MetaCell,
- MeSH
- alfa receptor estrogenů genetika MeSH
- dospělí MeSH
- genetická heterogenita * MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové biomarkery genetika MeSH
- nádorové cirkulující buňky patologie MeSH
- nádory prsu krev genetika patologie MeSH
- receptor erbB-2 genetika MeSH
- regulace genové exprese u nádorů MeSH
- senioři MeSH
- staging nádorů MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- alfa receptor estrogenů MeSH
- ERBB2 protein, human MeSH Prohlížeč
- ESR1 protein, human MeSH Prohlížeč
- nádorové biomarkery MeSH
- receptor erbB-2 MeSH
INTRODUCTION: This study analyzes peripheral blood samples from breast cancer (BC) patients. CTCs from peripheral blood were enriched by size-based separation and were then cultivated in vitro. The primary aim of this study was to demonstrate the antigen independent CTC separation method with high CTC recovery. Subsequently, CTCs enriched several times during the treatment were characterized molecularly. METHODS: Patients with different stages of BC (N = 167) were included into the study. All patients were candidates for surgery, surgical diagnostics, or were undergoing chemotherapy. In parallel, 20 patients were monitored regularly and in addition to CTC presence, also CTC character was examined by qPCR, with special focus on HER2 and ESR status. RESULTS: CTC positivity in the cohort was 76%. There was no significant difference between the tested groups, but the highest CTC occurrence was identified in the group undergoing surgery and similarly in the group before the start of neoadjuvant treatment. On the other hand, the lowest CTC frequencies were observed in the menopausal patient group (56%), ESR+ patient group (60%), and DCIS group (44.4%). It is worth noting that after completion of neoadjuvant therapy (NACT) CTCs were present in 77.7% of cases. On the other hand, patients under hormonal treatment were CTC positive only in 52% of cases. DISCUSSIONS: Interestingly, HER2 and ESR status of CTCs differs from the status of primary tumor. In 50% of patients HER2 status on CTCs changed not only from HER2+ to HER2-, but also from HER2- to HER2+ (33%). ESR status in CTCs changed only in one direction from ESR+ to ESR-. CONCLUSIONS: Data obtained from the present study suggest that BC is a heterogeneous disease but CTCs may be detected independently of the disease characteristics in 76% of patients at any time point during the course of the disease. This relatively high CTC occurrence in BC should be considered when planning the long-term patient monitoring.
