The common lavender (Lavandula angustifolia Mill.) pectic polysaccharides modulate phagocytic leukocytes and intestinal Peyer's patch cells
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
PubMed
28821152
DOI
10.1016/j.carbpol.2017.07.011
PII: S0144-8617(17)30777-4
Knihovny.cz E-resources
- Keywords
- Immunomodulation, Lavender, Macrophages, Neutrophils, Pectin, Peyer’s patches,
- MeSH
- Anti-Inflammatory Agents chemistry MeSH
- Rabbits MeSH
- Flowers chemistry MeSH
- Lavandula chemistry MeSH
- Humans MeSH
- Macrophages drug effects MeSH
- Mice, Inbred C3H MeSH
- Mice MeSH
- Sheep MeSH
- Nitric Oxide metabolism MeSH
- Pectins pharmacology MeSH
- Peyer's Patches cytology MeSH
- Polysaccharides MeSH
- RAW 264.7 Cells MeSH
- Reactive Oxygen Species metabolism MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Humans MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Anti-Inflammatory Agents MeSH
- Nitric Oxide MeSH
- Pectins MeSH
- Polysaccharides MeSH
- Reactive Oxygen Species MeSH
Two pectic (chPS-L1, chPS-L2) and one polyphenolic (chPP-L) fractions were obtained from lavender flowers after boiling water extraction, exhaustive removing of alcohol-soluble molecules and SEC. chPS-L1 (52.4kDa) contains mainly low-acetylated and high-methoxylated homogalacturonans (HG), and smaller rhamnogalacturonan (RG) I backbone fragments rich in 1,3,5-branched arabinan and arabinogalactan (AG) II side chains. chPS-L2 (21.8kDa) contains predominantly similarly esterified HG, followed by RGI with AGII structures and RGII. The prevalence of catechin and epicatechin in chPP-L indicates that they form weak interactions with pectins. chPS-L1 and chPS-L2 enhanced ß2-integrin expression on neutrophils, inducing ROS generation and macrophage NO production. Both the effects on ß2-integrin and high complement fixation activity of chPS-L1 were proposed for its inhibitory action against PMA- and OZP-activated ROS formation. This, together with suppression of NO generation after co-stimulation with chPS-L1 and LPS, suggested anti-inflammatory activity of studied pectins. Lavender polysaccharides expressed intestinal Peyer's patch immunomodulating activity.
Department of Biotechnology University of Food Technologies 26 Maritza Blvd BG 4002 Plovdiv Bulgaria
Department of Chemistry University of Oslo P O Box 1033 Blindern NO 0315 Oslo Norway
References provided by Crossref.org
