IrC2/Bf - A yeast and Borrelia responsive component of the complement system from the hard tick Ixodes ricinus
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
29061482
DOI
10.1016/j.dci.2017.10.012
PII: S0145-305X(17)30475-5
Knihovny.cz E-zdroje
- Klíčová slova
- Borrelia, C3-complement convertase, Factor B, Ixodes, Phagocytosis, Tick immunity,
- MeSH
- aktivace komplementu MeSH
- Borrelia burgdorferi imunologie MeSH
- Candida albicans imunologie MeSH
- fagocytóza MeSH
- hemocyty imunologie mikrobiologie MeSH
- hmyzí proteiny genetika metabolismus MeSH
- infekce přenášené vektorem MeSH
- interakce hostitele a patogenu MeSH
- kandidóza imunologie MeSH
- klíště imunologie MeSH
- komplement C3 genetika metabolismus MeSH
- lidé MeSH
- lymeská nemoc imunologie MeSH
- malá interferující RNA genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- hmyzí proteiny MeSH
- komplement C3 MeSH
- malá interferující RNA MeSH
Ticks possess components of a primordial complement system that presumably play a role in the interaction of the tick immune system with tick-borne pathogens and affect their transmission. Here we characterized a novel complement component, tagged as IrC2/Bf, from the hard tick Ixodes ricinus, the principal vector of Lyme disease in Europe. IrC2/Bf is a multi-domain molecule composed of 5-7 CCP modules, varied by alternative splicing, followed by a von Willebrand factor A domain and a C-terminal trypsin-like domain. The primary structure and molecular architecture of IrC2/Bf displays the closest homology to the C3-complement component convertases described in horseshoe crabs. The irc2/bf gene is mainly expressed in the tick fat body associated with the trachea and, as determined by western blotting, the protein is present in low amounts in tick hemolymph. Expression of irc2/bf mRNA was significantly up-regulated in response to the intra-hemocoelic injection of the yeast Candida albicans and all tested Borrelia sp. strains (B. burgdorferi NE5264, B. burgdorferi CB26, B. garinii MSLB, B. afzelii CB43), but was not affected by injection of model Gram-negative and Gram-positive bacteria or the aseptic injection control. In-line with these results, RNAi-mediated silencing of irc2/bf inhibited phagocytosis of B. afzelii and C. albicans but not the other bacteria. Tissue expression profiles, specific responses to microbial challenges, and patterns of phagocytic phenotypes upon RNAi silencing observed for IrC2/Bf match well with the previously reported characteristics of I. ricinus C3-related molecule 1 (IrC3-1). Therefore we presume that IrC2/Bf functions as a convertase in the same complement activation pathway protecting ticks against yeast and Borrelia infection.
Citace poskytuje Crossref.org
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