The present study is focused on the synthesis and investigation of the physicochemical and biological properties of silver nanoparticles stabilized with a series of cationic gemini surfactants having a polymethylene spacer of variable length. UV-VIS spectroscopy, dynamic light scattering, scanning electron microscopy and zeta potential measurements were applied to provide physicochemical characterization of the silver nanoparticles. The mean size values of the nanoparticles were found to be in the 50 to 115 nm range. From the nanoparticle size distributions and scanning electron microscopy images it results that a population of small nanoparticles with the size of several nanometers was confirmed if the nanoparticles were stabilized with gemini molecules with either a short methylene spacer (two or four -CH₂- groups) or a long spacer (12 -CH₂- groups). The average zeta potential value for silver nanoparticles stabilized with gemini molecules is roughly independent of gemini surfactant spacer length and is approx. +58 mV. An interaction model between silver nanoparticles and gemini molecules which reflects the gained experimental data, is suggested. Microbicidal activity determinations revealed that the silver nanoparticles stabilized with gemini surfactants are more efficient against Gram-negative bacteria and yeasts, which has a direct relation to the interaction mechanism of nanoparticles with the bacterial cell membrane and its structural composition.

Department of Cell and Molecular Biology of Drugs Faculty of Pharmacy Comenius University Bratislava SK 83232 Slovakia

Department of Chemical Theory of Drugs Faculty of Pharmacy Comenius University Kalinčiakova 8 Bratislava SK 83232 Slovakia

Department of Pharmaceutical Chemistry Faculty of Pharmacy Comenius University Bratislava SK 83232 Slovakia

Faculty of Pharmacy Comenius University Kalinčiakova 8 Bratislava SK 83232 Slovakia

Materials Research Centre Faculty of Chemistry Brno University of Technology CZ 61200 Brno Czech Republic

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