Identification of Plant Nuclear Proteins Based on a Combination of Flow Sorting, SDS-PAGE, and LC-MS/MS Analysis
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Cell cycle, Flow cytometry, Gel electrophoresis, In-gel digestion, Mass spectrometry, Nuclear proteome, Plant nucleus, Protein analysis,
- MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- jaderné proteiny analýza MeSH
- ječmen (rod) cytologie metabolismus MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- proteomika metody MeSH
- průtoková cytometrie MeSH
- rostlinné proteiny analýza MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- jaderné proteiny MeSH
- rostlinné proteiny MeSH
In the plant nucleus, the majority of cellular DNA content is stored and maintained. This makes this highly specialized organelle the major coordinator of almost all essential processes in plant cells such as transcription, DNA replication, and repair. None of these biological pathways can be fully understood without a comprehensive characterization of nuclear proteins. Nevertheless, the interest of the proteomic community in the plant nuclear proteome has been very limited so far. This is probably due to the high integrity of plant cell, presence of many interfering metabolites, and considerable endogenous proteolytic activity which make the sample preparation problematic. Hereby, we describe a novel protocol for the high-throughput plant nuclear protein identification that combines a flow cytometric sorting of formaldehyde-fixed nuclei with protein and peptide separation and their subsequent LC-MS/MS analysis.
Citace poskytuje Crossref.org
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