The impact of sesquiterpenes β-caryophyllene oxide and trans-nerolidol on xenobiotic-metabolizing enzymes in mice in vivo
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
- Keywords
- Cytochrome P450, drug-metabolizing enzyme, enzyme induction, terpenes,
- MeSH
- Aldehyde Reductase metabolism MeSH
- Enzymes metabolism MeSH
- Estradiol Dehydrogenases metabolism MeSH
- Mice, Inbred Strains MeSH
- Liver drug effects enzymology MeSH
- Inactivation, Metabolic drug effects MeSH
- NAD(P)H Dehydrogenase (Quinone) metabolism MeSH
- Polycyclic Sesquiterpenes MeSH
- Gene Expression Regulation, Enzymologic drug effects MeSH
- Sesquiterpenes pharmacology toxicity MeSH
- Cytochrome P-450 Enzyme System genetics metabolism MeSH
- Intestine, Small drug effects enzymology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Akr1a1 protein, mouse MeSH Browser
- AKR1C13 protein, mouse MeSH Browser
- Aldehyde Reductase MeSH
- caryophyllene oxide MeSH Browser
- Enzymes MeSH
- Estradiol Dehydrogenases MeSH
- NAD(P)H Dehydrogenase (Quinone) MeSH
- nerolidol MeSH Browser
- Nqo1 protein, mouse MeSH Browser
- Polycyclic Sesquiterpenes MeSH
- Sesquiterpenes MeSH
- Cytochrome P-450 Enzyme System MeSH
1. Sesquiterpenes, constituents of plant essential oil, are popular bioactive compounds due to the positive effect on human health, but their potential toxicity and possible herb-drug interactions are often omitted. In our in vivo study, we followed up the effect of p.o. administration of two sesquiterpenes β-caryophyllene oxide (CAO) and trans-nerolidol (NER) on various xenobiotic-metabolizing enzymes in mice liver and small intestine. 2. To spot the early effect of studied compounds, enzymatic activity and mRNA levels were assessed 6 and 24 h after single dose. 3. CAO and NER markedly increased cytochromes P450 (CYP2B, 3A, 2C) activity and mRNA levels in both tissues. Liver also showed elevated activity of aldo-ketoreductase 1C and carbonyl reductase after treatment. Contrary, sesquiterpenes decreased NAD(P)H:quinone oxidoreductase 1 activity in small intestine. Among conjugation enzymes, only liver sulfotransferase activity was increased by sesquiterpenes. 4. Our results document that single dose of sesquiterpenes modulate activities and expression of several xenobiotic-metabolizing enzymes.
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