Substances present in plants are used for therapeutic and cosmetic purposes. Some of them can however irritate the skin or hypersensitivity may develop. Potent plant sensitizers are present in oily resins, in particular in the group of volatile oils. The latter are a mixture of volatile and usually fragrant substances which among others contain terpenes. The latter are closely related to isoprene (2-methyl-1.3-butadiene). By combination of two or more isoprene units various terpenes are formed. Potent sensitizers are found in particular among mono- and sesquiterpenes and among them in particular the group of sesquiterpenic lactones is of interest under our conditions as it is the basis for the possible development of sensitization of the eczema type after contact with plants of the Asteraceae-Compositae family. To detect contact sensitization to sesquiterpenic lactones also commercially prepared allergen mixtures can be used („Sesquiterpene-lactone-Mix" 0.1% in petroleum jelly) which is part of the European standard series of Trolab allergens of Hermal Co.

• MeSH
• alergická kontaktní dermatitida etiologie   MeSH
• Asteraceae MeSH
• kožní testy MeSH
• lidé MeSH
• terpeny škodlivé účinky   MeSH
• Check Tag
• lidé MeSH

• MeSH
• antikarcinogenní látky MeSH
• kardiovaskulární látky terapeutické užití   MeSH
• lidé MeSH
• steroly metabolismus   terapeutické užití   MeSH
• terpeny metabolismus   terapeutické užití   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• přehledy MeSH

Advancements in small-molecule research have created the need for sensitive techniques to accurately study biological processes in living systems. Fluorescent-labeled probes have become indispensable tools, particularly those that use boron-dipyrromethene (BODIPY) dyes. Terpenes and terpenoids are organic compounds found in nature that offer diverse biological activities, and BODIPY-based probes play a crucial role in studying these compounds. Monoterpene-BODIPY conjugates have exhibited potential for staining bacterial and fungal cells. Sesquiterpene-BODIPY derivatives have been used to study sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA), indicating their potential for drug development. Owing to their unique properties, diterpenes have been investigated using BODIPY conjugates to evaluate their mechanisms of action. Triterpene-BODIPY conjugates have been synthesized for biological studies, with different spacers affecting their cytotoxicity. Fluorescent probes, inspired by terpenoid-containing vitamins, have also been developed. Derivatives of tocopherol, coenzyme Q10, and vitamin K1 can provide insights into their oxidation-reduction abilities. All these probes have diverse applications, including the study of cell membranes to investigate immune responses and antioxidant properties. Further research in this field can help better understand and use terpenes and terpenoids in various biological contexts.

• MeSH
• fluorescenční barviva chemie   MeSH
• lidé MeSH
• molekulární struktura MeSH
• sloučeniny boru * chemie   farmakologie   MeSH
• terpeny * chemie   farmakologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

An original method based on capillary zone electrophoresis with fluorimetric detection has been developed for the determination of terpenic compounds. The method is based on the separation of a terpenes dynamically labeled by the non-ionogenic tenside poly(ethylene glycol) pyrenebutanoate, which was used previously for the labeling of biopolymers. The background electrolytes were composed of taurine-Tris buffer (pH 8.4). In addition to the non-ionogenic tenside aceton and poly(ethylene glycol) were used as the additives. The capillary zone electrophoresis with fluorometric detection at the excitation wavelength 335 nm and the emission wavelength 463 nm was successfully applied to the analysis of tonalid, cholesterol, vitamin A, ergosterol, estrone and farnesol at level of 10(-17) mol L(-1). Farnesol, is produced by Candida albicans as an extracellular quorum-sensing molecule that influences expression of a number of virulence factors, especially morphogenesis and biofilm formation. It enables this yeast to cause serious nosocomial infections. The sensitivity of this method was demonstrated on the separation of farnesol directly from the cultivation medium.

• MeSH
• biofilmy MeSH
• butyráty chemie   MeSH
• Candida albicans chemie   metabolismus   MeSH
• cholesterol chemie   MeSH
• elektroforéza kapilární metody   MeSH
• ergosterol chemie   MeSH
• estron chemie   MeSH
• farnesol chemie   izolace a purifikace   metabolismus   MeSH
• fluorometrie metody   MeSH
• polymery chemie   MeSH
• quorum sensing MeSH
• senzitivita a specificita MeSH
• terpeny chemie   izolace a purifikace   MeSH
• tetrahydronaftaleny chemie   MeSH
• vitamin A chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• MeSH
• farnesol analogy a deriváty   MeSH
• feromony chemie   MeSH
• finanční podpora výzkumu jako téma MeSH
• terpeny chemie   MeSH
• včely chemie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH

Methods for the separation and determination of terpenes (mono- and sesqui-) and phenylpropanoids such as eugenol and methyleugenol from samples of allspice berries have been developed. Chromatographic analyses of isolated groups of compounds were carried out by means of gas chromatography coupled with mass spectrometry. A comparison of various types of solid-phase microextraction fibers was performed. The highest yields of terpenes were extracted by polydimethylsiloxane and divinylbenzene/Carboxen/polydimethylsiloxane fibers (almost the same for these two fibers), approximately twice as much as by Carbowax/divinylbenzene fiber. The highest amounts of monoterpenes were extracted by divinylbenzene/Carboxen/polydimethylsiloxane fiber, and the highest amounts of sesquiterpenes were extracted by polydimethylsiloxane fiber. Moreover, the effect of water addition on extraction yields as well as time and temperature of extraction were tested. Aroma profiles of extracts obtained by solid-phase microextraction and essential oil obtained by hydrodistillation of allspice berries were compared. The aroma profile of the divinylbenzene/Carboxen/polydimethylsiloxane fiber extract was similar to the aroma profile of essential oil. Particular characteristics of volatile allspice matters were presented. The linear retention indices for each compound were calculated.

• MeSH
• destilace MeSH
• dimethylpolysiloxany chemie   MeSH
• mikroextrakce na pevné fázi metody   MeSH
• monoterpeny chemie   MeSH
• oleje prchavé MeSH
• Pimenta (rod) chemie   MeSH
• plynová chromatografie s hmotnostně spektrometrickou detekcí metody   MeSH
• polyethylenglykoly chemie   MeSH
• seskviterpeny chemie   MeSH
• teplota MeSH
• terpeny analýza   chemie   MeSH
• vinylové sloučeniny chemie   MeSH
• voda chemie   MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• biologické přípravky MeSH
• fyziologie rostlin MeSH
• rostliny MeSH
• terpeny MeSH
• znečištění životního prostředí MeSH
• Publikační typ
• srovnávací studie MeSH

New quaternary ammonium salt-type compounds with lipophilic cholesterol and terpene moieties were synthesized. The compounds showed promising antibacterial and antimycobacterial activities. Those compounds containing the cholesterol moiety showed significant activity against Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecium. On the contrary, the antimycobacterial activity increased with the presence of the terpene unit in the molecule.

• MeSH
• antiinfekční látky chemická syntéza   farmakologie   MeSH
• cholesterol analogy a deriváty   chemická syntéza   farmakologie   MeSH
• Enterococcus faecium účinky léků   růst a vývoj   MeSH
• houby účinky léků   růst a vývoj   MeSH
• kvartérní amoniové sloučeniny chemická syntéza   farmakologie   MeSH
• mikrobiální testy citlivosti MeSH
• molekulární struktura MeSH
• Mycobacterium účinky léků   růst a vývoj   MeSH
• racionální návrh léčiv MeSH
• Staphylococcus aureus účinky léků   růst a vývoj   MeSH
• Staphylococcus epidermidis účinky léků   růst a vývoj   MeSH
• terpeny diagnostické užití   farmakologie   MeSH
• vztahy mezi strukturou a aktivitou MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH

The current study explored the in vitro anticancer properties of Mesua ferrea stem bark (SB) extract towards human colon carcinoma HCT116 cells. SB was successively extracted with different solvents using soxhlet apparatus. MTT assay was employed to test toxicity against different cancer and normal cell lines. Active extract (n-Hexane) was fractionated by column chromatography (CC) to get the most active fraction (F-3). Series of in vitro assays were employed to characterize cytotoxic nature of F-3. Antioxidant properties of F-3 were assessed using DPPH, ABTS and FRAP assays followed by GC–MS analysis. Intracellular ROS levels were measured by DCFH-DA fluorescent assay. Finally, cell signalling pathways and their downstream proteins targeted by F-3 were studied using 10-cancer pathway and human apoptosis protein profilers and in silico docking studies. n-Hexane extract and its fraction (F-3) showed potent anti-proliferative effect against HCT 116. Programmed cell death (PCD) studies showed that F-3 modulated the expression of multiple proteins in HCT 116. F-3 showed weak antioxidant activity in all the models, while significant increase in ROS was observed in HCT 116. GC–MS analysis revealed that F-3 was majorly comprised of terpenes. Data of pathway profiler and in silico studies revealed that F-3 down-regulated the expression of NF-κB and HIF-1α pathways. Overall these results demonstrate that anticancer effects of M. ferrea stem bark towards human colon carcinoma are mainly due to its terpenes contents.

• MeSH
• adaptorové proteiny signální transdukční MeSH
• apoptóza účinky léků   MeSH
• buněčná smrt účinky léků   MeSH
• buňky NK účinky léků   ultrastruktura   MeSH
• kolorektální nádory farmakoterapie   genetika   patologie   MeSH
• Malpighiaceae chemie   MeSH
• nádorové buněčné linie účinky léků   MeSH
• rostlinné extrakty farmakologie   terapeutické užití   MeSH
• techniky in vitro MeSH
• terpeny farmakologie   terapeutické užití   MeSH
• Publikační typ
• práce podpořená grantem MeSH

• MeSH
• bronchokonstrikce účinky léků   MeSH
• krysa rodu rattus MeSH
• kyanatany škodlivé účinky   MeSH
• kyselina arachidonová metabolismus   MeSH
• morčata MeSH
• oxid siřičitý škodlivé účinky   MeSH
• plíce účinky léků   MeSH
• terpeny škodlivé účinky   MeSH
• thromboxany metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• morčata MeSH
• zvířata MeSH