Characterization of KPC-Encoding Plasmids from Enterobacteriaceae Isolated in a Czech Hospital
Language English Country United States Media electronic-print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
29263065
PubMed Central
PMC5826142
DOI
10.1128/aac.02152-17
PII: AAC.02152-17
Knihovny.cz E-resources
- Keywords
- Citrobacter freundii, Illumina sequencing, IncR, ST18, Tn4401a,
- MeSH
- Anti-Bacterial Agents therapeutic use MeSH
- beta-Lactamases genetics metabolism MeSH
- Citrobacter freundii drug effects enzymology genetics isolation & purification MeSH
- Enterobacteriaceae Infections drug therapy epidemiology microbiology MeSH
- Escherichia coli drug effects enzymology genetics isolation & purification MeSH
- Gene Expression MeSH
- Isoenzymes genetics metabolism MeSH
- Carbapenems therapeutic use MeSH
- Klebsiella pneumoniae drug effects enzymology genetics isolation & purification MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Drug Resistance, Multiple, Bacterial genetics MeSH
- Morganella morganii drug effects enzymology genetics isolation & purification MeSH
- Hospitals MeSH
- Open Reading Frames MeSH
- Plasmids chemistry classification metabolism MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- DNA Transposable Elements MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- beta-lactamase KPC-2 MeSH Browser
- beta-Lactamases MeSH
- Isoenzymes MeSH
- Carbapenems MeSH
- DNA Transposable Elements MeSH
Ten Enterobacteriaceae isolates collected in a Czech hospital carried blaKPC-positive plasmids of different sizes (∼30, ∼45, and ∼80 kb). Sequencing revealed three types of plasmids (A to C) with the Tn4401a transposon. Type A plasmids comprised an IncR backbone and a KPC-2-encoding multidrug resistance (MDR) region. Type B plasmids were derivatives of type A plasmids carrying an IncN3-like segment, while type C plasmids were IncP6 plasmids sharing the same KPC-2-encoding MDR region with type A and B plasmids.
Biomedical Center Faculty of Medicine Charles University Pilsen Czech Republic
Department of Hospital Hygiene University Hospital Hradec Kralove Czech Republic
National Centre for Biomolecular Research Faculty of Science Masaryk University Brno Czech Republic
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