Injectable hydroxyphenyl derivative of hyaluronic acid hydrogel modified with RGD as scaffold for spinal cord injury repair
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
29266693
DOI
10.1002/jbm.a.36311
Knihovny.cz E-resources
- Keywords
- hyaluronic acid, mesenchymal stem cells, regenerative medicine, scaffold, spinal cord injury,
- MeSH
- Hydrogels chemistry MeSH
- Injections * MeSH
- Hyaluronic Acid chemistry MeSH
- Humans MeSH
- RNA, Messenger genetics metabolism MeSH
- Mesenchymal Stem Cells cytology drug effects MeSH
- Oligopeptides chemistry MeSH
- Motor Activity MeSH
- Spinal Cord Injuries pathology physiopathology MeSH
- Rats, Wistar MeSH
- Spinal Cord Regeneration * MeSH
- Tissue Scaffolds chemistry MeSH
- Wharton Jelly cytology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- arginyl-glycyl-aspartic acid MeSH Browser
- Hydrogels MeSH
- Hyaluronic Acid MeSH
- RNA, Messenger MeSH
- Oligopeptides MeSH
Hydrogel scaffolds which bridge the lesion, together with stem cell therapy represent a promising approach for spinal cord injury (SCI) repair. In this study, a hydroxyphenyl derivative of hyaluronic acid (HA-PH) was modified with the integrin-binding peptide arginine-glycine-aspartic acid (RGD), and enzymatically crosslinked to obtain a soft injectable hydrogel. Moreover, addition of fibrinogen was used to enhance proliferation of human Wharton's jelly-derived mesenchymal stem cells (hWJ-MSCs) on HA-PH-RGD hydrogel. The neuroregenerative potential of HA-PH-RGD hydrogel was evaluated in vivo in acute and subacute models of SCI. Both HA-PH-RGD hydrogel injection and implantation into the acute spinal cord hemisection cavity resulted in the same axonal and blood vessel density in the lesion area after 2 and 8 weeks. HA-PH-RGD hydrogel alone or combined with fibrinogen (HA-PH-RGD/F) and seeded with hWJ-MSCs was then injected into subacute SCI and evaluated after 8 weeks using behavioural, histological and gene expression analysis. A subacute injection of both HA-PH-RGD and HA-PH-RGD/F hydrogels similarly promoted axonal ingrowth into the lesion and this effect was further enhanced when the HA-PH-RGD/F was combined with hWJ-MSCs. On the other hand, no effect was found on locomotor recovery or the blood vessel ingrowth and density of glial scar around the lesion. In conclusion, we have developed and characterized injectable HA-PH-RGD based hydrogel, which represents a suitable material for further combinatorial therapies in neural tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1129-1140, 2018.
2nd Medical Faculty Charles University Prague Czech Republic
Department of Tissue Engineering Contipro a s Dolni Dobrouc Czech Republic
References provided by Crossref.org
Mesenchymal Stem Cells in Treatment of Spinal Cord Injury and Amyotrophic Lateral Sclerosis
Modified Methacrylate Hydrogels Improve Tissue Repair after Spinal Cord Injury
