Injectable hydroxyphenyl derivative of hyaluronic acid hydrogel modified with RGD as scaffold for spinal cord injury repair
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
29266693
DOI
10.1002/jbm.a.36311
Knihovny.cz E-zdroje
- Klíčová slova
- hyaluronic acid, mesenchymal stem cells, regenerative medicine, scaffold, spinal cord injury,
- MeSH
- hydrogely chemie MeSH
- injekce * MeSH
- kyselina hyaluronová chemie MeSH
- lidé MeSH
- messenger RNA genetika metabolismus MeSH
- mezenchymální kmenové buňky cytologie účinky léků MeSH
- oligopeptidy chemie MeSH
- pohybová aktivita MeSH
- poranění míchy patologie patofyziologie MeSH
- potkani Wistar MeSH
- regenerace míchy * MeSH
- tkáňové podpůrné struktury chemie MeSH
- Whartonův rosol cytologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- arginyl-glycyl-aspartic acid MeSH Prohlížeč
- hydrogely MeSH
- kyselina hyaluronová MeSH
- messenger RNA MeSH
- oligopeptidy MeSH
Hydrogel scaffolds which bridge the lesion, together with stem cell therapy represent a promising approach for spinal cord injury (SCI) repair. In this study, a hydroxyphenyl derivative of hyaluronic acid (HA-PH) was modified with the integrin-binding peptide arginine-glycine-aspartic acid (RGD), and enzymatically crosslinked to obtain a soft injectable hydrogel. Moreover, addition of fibrinogen was used to enhance proliferation of human Wharton's jelly-derived mesenchymal stem cells (hWJ-MSCs) on HA-PH-RGD hydrogel. The neuroregenerative potential of HA-PH-RGD hydrogel was evaluated in vivo in acute and subacute models of SCI. Both HA-PH-RGD hydrogel injection and implantation into the acute spinal cord hemisection cavity resulted in the same axonal and blood vessel density in the lesion area after 2 and 8 weeks. HA-PH-RGD hydrogel alone or combined with fibrinogen (HA-PH-RGD/F) and seeded with hWJ-MSCs was then injected into subacute SCI and evaluated after 8 weeks using behavioural, histological and gene expression analysis. A subacute injection of both HA-PH-RGD and HA-PH-RGD/F hydrogels similarly promoted axonal ingrowth into the lesion and this effect was further enhanced when the HA-PH-RGD/F was combined with hWJ-MSCs. On the other hand, no effect was found on locomotor recovery or the blood vessel ingrowth and density of glial scar around the lesion. In conclusion, we have developed and characterized injectable HA-PH-RGD based hydrogel, which represents a suitable material for further combinatorial therapies in neural tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1129-1140, 2018.
2nd Medical Faculty Charles University Prague Czech Republic
Department of Tissue Engineering Contipro a s Dolni Dobrouc Czech Republic
Citace poskytuje Crossref.org
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