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PubMed

Záznam pochází z PubMed

First delivery and ovariectomy affect biomechanical and structural properties of the vagina in the ovine model

Urbankova, Iva
Autor Urbankova, Iva Centre for Surgical Technologies, Faculty of Medicine, KU Leuven, Leuven, Belgium Department of Development and Regeneration, Clinical Specialties Research Groups, Faculty of Medicine, KU Leuven, Leuven, Belgium Institute for the Care of Mother and Child and Third Faculty of Medicine, Charles University, Prague, Czech Republic
Callewaert, Geertje
Autor Callewaert, Geertje Centre for Surgical Technologies, Faculty of Medicine, KU Leuven, Leuven, Belgium Department of Development and Regeneration, Clinical Specialties Research Groups, Faculty of Medicine, KU Leuven, Leuven, Belgium Pelvic Floor Unit, Departments of Obstetrics & Gynecology and Urology, University Hospitals KU Leuven, Leuven, Belgium
Blacher, Silvia
Autor Blacher, Silvia Laboratory of Tumor and Development Biology, GIGA-Cancer, Institute of Pathology, University of Liège, Liège, Belgium
Deprest, Dries
Autor Deprest, Dries Centre for Surgical Technologies, Faculty of Medicine, KU Leuven, Leuven, Belgium Department of Development and Regeneration, Clinical Specialties Research Groups, Faculty of Medicine, KU Leuven, Leuven, Belgium
Hympanova, Lucie
Autor Hympanova, Lucie Centre for Surgical Technologies, Faculty of Medicine, KU Leuven, Leuven, Belgium Department of Development and Regeneration, Clinical Specialties Research Groups, Faculty of Medicine, KU Leuven, Leuven, Belgium Institute for the Care of Mother and Child and Third Faculty of Medicine, Charles University, Prague, Czech Republic
Feola, Andrew
Autor Feola, Andrew Centre for Surgical Technologies, Faculty of Medicine, KU Leuven, Leuven, Belgium Department of Development and Regeneration, Clinical Specialties Research Groups, Faculty of Medicine, KU Leuven, Leuven, Belgium
De Landsheere, Laurent
Autor De Landsheere, Laurent Department of Obstetrics and Gynaecology, University of Liège, Liège, Belgium
Deprest, Jan
Autor Deprest, Jan Department of Development and Regeneration, Clinical Specialties Research Groups, Faculty of Medicine, KU Leuven, Leuven, Belgium. Jan.Deprest@uzleuven.be Pelvic Floor Unit, Departments of Obstetrics & Gynecology and Urology, University Hospitals KU Leuven, Leuven, Belgium. Jan.Deprest@uzleuven.be Institute for Women's Health, University College London, London, UK. Jan.Deprest@uzleuven.be Department of Development and Regeneration, Group Biomedical Sciences, KU Leuven, Herestraat 49 box 7003, 3000, Leuven, Belgium. Jan.Deprest@uzleuven.be

International urogynecology journal. 2019 Mar ; 30 (3) : 455-464. [epub] 20180108

Int Urogynecol J
ISSN 1433-3023 | 0937-3462
Zdroj

Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic

Typ dokumentu časopisecké články

Perzistentní odkaz https://www.medvik.cz/link/pmid29313089

PubMed 29313089
DOI 10.1007/s00192-017-3535-9
PII: 10.1007/s00192-017-3535-9
Knihovny.cz E-zdroje

Klíčová slova
Animal model, Biomechanics, Contractility, Sheep vagina, Vaginal delivery,
MeSH
aktiny metabolismus MeSH
alfa receptor estrogenů metabolismus MeSH
biomechanika MeSH
elastin metabolismus MeSH
epitel metabolismus patologie MeSH
glykogen metabolismus MeSH
hladké svalstvo patofyziologie MeSH
hormonální substituční terapie MeSH
kolagen metabolismus MeSH
modely nemocí na zvířatech MeSH
onemocnění dna pánevního etiologie MeSH
ovarektomie škodlivé účinky MeSH
ovce MeSH
parita MeSH
porod * MeSH
svalová kontrakce MeSH
vagina patologie patofyziologie MeSH
zvířata MeSH
Check Tag
ženské pohlaví MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
Názvy látek
ACTA2 protein, human MeSH Prohlížeč
aktiny MeSH
alfa receptor estrogenů MeSH
elastin MeSH
glykogen MeSH
kolagen MeSH

INTRODUCTION AND HYPOTHESIS: Animal models are useful for investigating the genesis of pelvic floor dysfunction and for developing novel therapies for its treatment. There is a need for an alternative large-animal model to the nonhuman primate. Therefore we studied the effects of the first vaginal delivery, ovariectomy and systemic hormonal replacement therapy (HRT) on the biomechanical and structural properties of the ovine vagina. METHODS: We examined the gross anatomical properties of nulliparous, primiparous, ovariectomized multiparous, and ovariectomized hormone-replaced multiparous sheep (six animals per group). We also harvested mid-vaginal and distal vaginal tissue to determine smooth muscle contractility and passive biomechanical properties, for morphometric assessment of the vaginal wall layers, to determine collagen and elastin content, and for immunostaining for α-smooth muscle actin and estrogen receptor-α. RESULTS: There were no regional differences in the nulliparous vagina. One year after the first vaginal delivery, stiffness and contractility of the distal vagina were decreased, whereas the elastin content increased. The mid-vagina of ovariectomized sheep was stiff, and its epithelium was thin and lacked glycogen. HRT decreased the stiffness of the mid-vagina by 45% but had no measurable effect on contractility or elastin content, and increased epithelial thickness and glycogen content. HRT also increased the epithelial thickness and glycogen content of the distal vagina. At this location, there were no changes in morphology or stiffness. CONCLUSION: In sheep, life events including delivery and ovariectomy affect the biomechanical properties of the vagina in a region-specific way. Vaginal delivery mainly affects the distal region by decreasing stiffness and contractility. HRT can reverse the increase in stiffness of the mid-vagina observed after surgical induction of menopause. These observations are in line with scanty biomechanical measurements in comparable clinical specimens.

Centre for Surgical Technologies Faculty of Medicine KU Leuven Leuven Belgium

Department of Development and Regeneration Clinical Specialties Research Groups Faculty of Medicine KU Leuven Leuven Belgium

Department of Development and Regeneration Group Biomedical Sciences KU Leuven Herestraat 49 box 7003 3000 Leuven Belgium

Department of Obstetrics and Gynaecology University of Liège Liège Belgium

Institute for the Care of Mother and Child and 3rd Faculty of Medicine Charles University Prague Czech Republic

Institute for Women's Health University College London London UK

Laboratory of Tumor and Development Biology GIGA Cancer Institute of Pathology University of Liège Liège Belgium

Pelvic Floor Unit Departments of Obstetrics and Gynecology and Urology University Hospitals KU Leuven Leuven Belgium

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